Minimum Information about a Tailoring Enzyme Repository

MITE0000400

MITE0000401

MITE0000402

Modify entry

Reaction Information

Number of Known Reactions: 1

Reaction 1

Description	Intramolecular nuclophilic attack of α-C eliminating the chlor-substituent on γ-C
Tailoring	Cyclization
Database References	No database crosslinks available
Evidence	Heterologous expression In vitro assay
References	doi:10.1038/nature03797 doi:10.1021/bi061930j

Example 1

Metadata	Description: Cyclization step forming coronamyl-S-CmaD Intermediate: True
Substrate 1
Product 1

Enzyme Information

Accession ID	MITE0000401
Entry Status	Status: active
Enzyme Name	CmaC
Enzyme Description	Cyclopropane-forming enzyme
Database References	UniProtKB: Q87W54 NCBI GenPept: AAO58150.1
References	doi:10.1021/bi061930j doi:10.1038/nature03797
Comment	Cyclopropanforming catalyst of γ-chloro-l-allo-Isoleucine intermediate in the coronatine pathway. See also MIBiG BGC0000328. Addition of Zn2+ increases enzyme activity.
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2026-06-23)

Contributor(s)	0009-0000-8651-1669
Reviewer(s)	0000-0001-6534-6609
Comment	"New entry. Reviewer 1 tried to replace structures but validation engine does not support chiral structures."