{
    "accession": "MITE0000401",
    "status": "active",
    "comment": "Cyclopropanforming catalyst of \u03b3-chloro-l-allo-Isoleucine intermediate in the coronatine pathway. See also MIBiG BGC0000328. Addition of Zn2+ increases enzyme activity.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-06-23",
            "contributors": [
                "0009-0000-8651-1669"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 1 tried to replace structures but validation engine does not support chiral structures."
        }
    ],
    "enzyme": {
        "name": "CmaC",
        "description": "Cyclopropane-forming enzyme",
        "references": [
            "doi:10.1021/bi061930j",
            "doi:10.1038/nature03797"
        ],
        "databaseIds": {
            "uniprot": "Q87W54",
            "genpept": "AAO58150.1"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Cyclization"
            ],
            "description": "Intramolecular nuclophilic attack of \u03b1-C eliminating the chlor-substituent on \u03b3-C",
            "reactionSMARTS": "[#16:1](-[*])-[#6:2](-[#6:4](-[#6:6](-[#6:9]-[Cl])-[#6:7]-[#6:8])-[#7:5])=[#8:3]>>[#16:1](-[*])-[#6:2](-[#6:4]1(-[#6:9]-[#6:6]-1-[#6:7]-[#6:8])-[#7:5])=[#8:3]",
            "reactions": [
                {
                    "substrate": "*SC(=O)C(N)C(CC)CCl",
                    "products": [
                        "*SC(=O)C1(N)CC1CC"
                    ],
                    "isIntermediate": true,
                    "description": "Cyclization step forming coronamyl-S-CmaD"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1038/nature03797",
                    "doi:10.1021/bi061930j"
                ]
            }
        }
    ]
}