Minimum Information about a Tailoring Enzyme Repository

MITE0000410

MITE0000411

MITE0000412

Modify entry

Reaction Information

Number of Known Reactions: 1

Reaction 1

Description	Oxidation of a methyl group to yield carboxylated functional group.
Tailoring	Oxidation
Database References	No database crosslinks available
Evidence	Inference from genomic data and chemical structure Knock-out studies
References	doi:10.1007/s00253-004-1802-4 doi:10.1016/j.chembiol.2008.08.009

Example 1

Metadata	Description: Oxidation of nystatin polyene scaffold to yield 16-carboxylated intermediate Intermediate: True
Substrate 1
Product 1

Enzyme Information

Accession ID	MITE0000411
Entry Status	Status: active
Enzyme Name	NysN
Enzyme Description	P450 monooxygenase
Database References	UniProtKB: Q9L4W8 NCBI GenPept: AAF71771.1 MIBiG: BGC0000115
Cofactors	Organic Heme
References	doi:10.1007/s00253-004-1802-4 doi:10.1016/j.chembiol.2008.08.009
Comment	Catalyzes oxidation of a methyl group in nystatin biosynthesis.
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2026-04-14)

Contributor(s)	0000-0001-5730-3326
Reviewer(s)	0000-0003-4819-2251
Comment	"New entry. Reviewer 1 fixed reaction SMARTS and substrate/product SMILES, added cofactor information, added reference and evidence qualifier."