{
    "accession": "MITE0000411",
    "status": "active",
    "comment": "Catalyzes oxidation of a methyl group in nystatin biosynthesis.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-14",
            "contributors": [
                "0000-0001-5730-3326"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "New entry. Reviewer 1 fixed reaction SMARTS and substrate/product SMILES, added cofactor information, added reference and evidence qualifier."
        }
    ],
    "enzyme": {
        "name": "NysN",
        "description": "P450 monooxygenase",
        "references": [
            "doi:10.1007/s00253-004-1802-4",
            "doi:10.1016/j.chembiol.2008.08.009 "
        ],
        "databaseIds": {
            "uniprot": "Q9L4W8",
            "genpept": "AAF71771.1",
            "mibig": "BGC0000115"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Oxidation"
            ],
            "description": "Oxidation of a methyl group to yield carboxylated functional group.",
            "reactionSMARTS": "[#6@:1]1(/[#8:48])-[#6@@:3](\\[#6:50])-[#6@:4](\\[#6:51])-[#8:5]-[#6:6](-[#6:8]-[#6@:9](/[#8:12])-[#6:10]-[#6@:11](/[#8:16])-[#6:13]-[#6@:14](/[#8:17])-[#6:15]-[#6:18]-[#6:19]-[#6@@:20](-[#6:22]-[#6@:23]2(/[#8:49])-[#6:25]-[#6@@:26](-[#6@:28](-[#6@@:52](\\[#8:24]-2)-[#6:29]-[#6@:31](-[#6:33]=[#6:34]-[#6:35]=[#6:36]-[#6:37]=[#6:38]-[#6:39]=[#6:40]-[#6:41]-[#6:42]-[#6:43]=[#6:44]-[#6:45]=[#6:46]-[#6@@:2]-1\\[#6:47])/[#8:32])/[#6:30])\\[#8:27])/[#8:21])=[#8:7]>>[#6@:1]1(/[#8:48])-[#6@@:3](\\[#6:50])-[#6@:4](\\[#6:51])-[#8:5]-[#6:6](-[#6:8]-[#6@:9](/[#8:12])-[#6:10]-[#6@:11](/[#8:16])-[#6:13]-[#6@:14](/[#8:17])-[#6:15]-[#6:18]-[#6:19]-[#6@@:20](-[#6:22]-[#6@:23]2(/[#8:49])-[#6:25]-[#6@@:26](-[#6@:28](-[#6@@:52](\\[#8:24]-2)-[#6:29]-[#6@:31](-[#6:33]=[#6:34]-[#6:35]=[#6:36]-[#6:37]=[#6:38]-[#6:39]=[#6:40]-[#6:41]-[#6:42]-[#6:43]=[#6:44]-[#6:45]=[#6:46]-[#6@@:2]-1\\[#6:47])/[#8:32])/[#6:30](-[#8])=[#8])\\[#8:27])/[#8:21])=[#8:7]",
            "reactions": [
                {
                    "substrate": "C[C@@H]1[C@H](O)[C@@H](C)/C=C/C=C/CC/C=C/C=C/C=C/C=C/[C@H](O)C[C@@H]2O[C@](O)(C[C@@H](O)CCC[C@@H](O)C[C@@H](O)C[C@@H](O)CC(=O)O[C@H]1C)C[C@H](O)[C@H]2C",
                    "products": [
                        "C[C@@H]1[C@H](O)[C@@H](C)C=CC=CCCC=CC=CC=CC=C[C@H](O)C[C@@H]2O[C@](O)(C[C@@H](O)CCC[C@@H](O)C[C@@H](O)C[C@@H](O)CC(=O)O[C@H]1C)C[C@H](O)[C@H]2C(=O)O"
                    ],
                    "isIntermediate": true,
                    "description": "Oxidation of nystatin polyene scaffold to yield 16-carboxylated intermediate"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Inference from genomic data and chemical structure",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1007/s00253-004-1802-4",
                    "doi:10.1016/j.chembiol.2008.08.009 "
                ]
            }
        }
    ]
}