MITE0000404
MITE0000405
MITE0000406
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Reaction Information

Number of Known Reactions: 3

Reaction 1
reaction SMARTS
Description Bicyclic halogenation through C5 radical intermediate product in the hectochlorin biosynthetic pathway.
Tailoring
  • Halogenation
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • In vitro assay
References
Example 1
Metadata
  • Description: C5 dichlorination of fatty acyl substrate.
  • Intermediate: True
Substrate 1 substrate SMILES
Product 1 product SMILES
Reaction 2
reaction SMARTS
Description Bicyclic halogenation and dehydrogenation through C5 radical intermediate product in the hectochlorin biosynthetic pathway.
Tailoring
  • Dehydrogenation
  • Halogenation
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • In vitro assay
References
Example 1
Metadata
  • Description: Single C5 chlorination and C4, C5 dehydrogenation resulting in a vinyl-chloride product of fatty acyl substrate.
  • Intermediate: True
Substrate 1 substrate SMILES
Product 1 product SMILES
Reaction 3
reaction SMARTS
Description Bicyclic monooxygenation through C5 radical intermediate product in the hectochlorin biosynthetic pathway.
Tailoring
  • Monooxygenation
  • Oxidation
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • In vitro assay
References
Example 1
Metadata
  • Description: C5 monoxygenation of fatty acyl substrate, resulting in keto-moiety.
  • Intermediate: True
Substrate 1 substrate SMILES
Product 1 product SMILES

Enzyme Information

Accession IDMITE0000405
Entry StatusStatus: active
Enzyme NameHctB
Enzyme DescriptionFatty acyl halogenase
Database References
Cofactors
  • Organic
    • a-Ketoglutaric acid
  • Inorganic
    • Fe
References
CommentMononuclear non-heme iron enzyme with high substrate- and reaction promiscuity, including halogenation (Cl, Br), oxygenation, and vinyl-chloride formation. Consists of unusual three-domain structure with N-terminal catalytic halogenase domain, an ACBP, and an substrate-anchoring ACP.
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2026-06-10)

Contributor(s)
Reviewer(s)
Comment "New entry."