Minimum Information about a Tailoring Enzyme Repository

MITE0000460

MITE0000461

MITE0000462

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Reaction Information

Number of Known Reactions: 2

Reaction 1

Description	Hydroxylation of narbomycin to form pikromycin
Tailoring	Hydroxylation
Database References	No database crosslinks available
Evidence	In vitro assay Knock-out studies
References	doi:10.1016/S1074-5521(98)90293-9

Example 1

Metadata	Description: Hydroxylation of narbomycin to form pikromycin Intermediate: True
Substrate 1
Product 1

Reaction 2

Description	Hydroxylation of YC-17 (10-Deoxymethymycin) to form methymycin
Tailoring	Hydroxylation
Database References	No database crosslinks available
Evidence	In vitro assay Knock-out studies
References	doi:10.1016/S1074-5521(98)90293-9

Example 1

Metadata	Description: Hydroxylation of YC-17 (10-Deoxymethymycin) to form methymycin Intermediate: True
Substrate 1
Product 1

Enzyme Information

Accession ID	MITE0000461
Entry Status	Status: active
Enzyme Name	PikC
Enzyme Description	Cytochrome P450
Database References	UniProtKB: O87605 NCBI GenPept: AAC64105.1
Cofactors	Organic Heme Inorganic Fe
References	doi:10.1073/pnas.95.21.12111 doi:10.1016/S1074-5521(98)90293-9 doi:10.1074/jbc.M605478200
Comment	Was previously associated to MIBiG BGC BGC0000898 but unclear which enzyme is referenced.
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2026-05-06)

Contributor(s)	0000-0001-9487-1525
Reviewer(s)	0000-0003-3290-1017 0000-0001-6534-6609
Comment	"New entry. Reviewer 2 moved MIBIG reference to comment due to lack of clear connection to a specific enzyme in the BGC."