MITE0000449
MITE0000450
MITE0000451
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Reaction Information

Number of Known Reactions: 1

Reaction 1
reaction SMARTS
Description 4′-O-methylation of 4′-demethylrebeccamycin to rebeccamycin using SAM as the methyl donor.
Tailoring
  • Methylation
Database References
Evidence
  • Heterologous expression
  • In vitro assay
  • Knock-out studies
  • Site-directed mutagenesis
References
Example 1
Metadata
  • Description: 4′-O-methylation of 4′-demethylrebeccamycin to rebeccamycin using SAM as the methyl donor.
  • Intermediate: False
Substrate 1 substrate SMILES
Product 1 product SMILES

Enzyme Information

Accession IDMITE0000450
Entry StatusStatus: active
Enzyme NameRebM
Enzyme DescriptionSAM-dependent O-methyltransferase
Database References
Cofactors
  • Organic
    • SAM
References
CommentRebM is the rebeccamycin sugar 4′-O-methyltransferase that catalyzes the final tailoring step in rebeccamycin biosynthesis. It transfers a methyl group from S-adenosyl-L-methionine to the 4′-hydroxy group of 4′-demethylrebeccamycin, forming rebeccamycin and S-adenosyl-L-homocysteine.
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2026-05-15)

Contributor(s)
Reviewer(s)
Comment "New entry. Reviewer 1 fixed ambiguous stereochemistry in smarts."