Reaction 1

• Reaction SMARTS
• Reaction Information

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Description	The last step in the biosynthesis of nigericin is the hydroxylation of the 30th carbon by NigD
Tailoring	Hydroxylation
Database References	No database crosslinks available
Evidence	Inference from genomic data and chemical structure In vitro assay Site-directed mutagenesis
References	doi:10.1016/j.chembiol.2007.05.011 doi:10.1021/acs.jafc.5c12869 doi:10.1016/j.nbt.2025.02.002

Example 1

Metadata	Description: An inhibitor of cytochrome P450 mono-oxygenases led to the accumulation of grisorixin (C-30-dehydroxylnigericin) and overexpression of nigD variant (mimicking demalonylation) increases nigericin production. Intermediate: False
Substrate 1	Copy SMILES
Product 1	Copy SMILES