MITE0000412
MITE0000413
MITE0000414
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Reaction Information

Number of Known Reactions: 3

Reaction 1
reaction SMARTS
Description Itarate dichlorination of tiacumicin B precourser
Tailoring
  • Halogenation
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • In vitro assay
  • Knock-out studies
References
Example 1
Metadata
  • Description: Dichlorination of homo-orsellinic acid moiety
  • Intermediate: False
Substrate 1 substrate SMILES
Product 1 product SMILES
Reaction 2
reaction SMARTS
Description Itarate chlorination followed by a bromination of tiacumicin B precourser
Tailoring
  • Halogenation
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • In vitro assay
  • Knock-out studies
References
Example 1
Metadata
  • Description: Chlorination and bromination of homo-orsellinic acid moiety
  • Intermediate: False
Substrate 1 substrate SMILES
Product 1 product SMILES
Reaction 3
reaction SMARTS
Description Itarate dibromination of tiacumicin B precourser
Tailoring
  • Halogenation
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • In vitro assay
  • Knock-out studies
References
Example 1
Metadata
  • Description: Dibromination of homo-orsellinic acid moiety
  • Intermediate: False
Substrate 1 substrate SMILES
Product 1 product SMILES

Enzyme Information

Accession IDMITE0000413
Entry StatusStatus: active
Enzyme NameTiaM
Enzyme DescriptionFlavin-dependent halogenase
Database References
Cofactors
  • Organic
    • FAD
    • NAD
References
CommentIterate halogenation of a substrate named "compund 9" defined as unchlorinated tiacumicin B in the tiacumicin B pathway. Non subtrate: free standing orsellinic Acid, I, F. The flavin auxiliary enzyme is no itdentified yet, therefore flavin-reductase SsuE gen was expressed instead.
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2026-07-02)

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Reviewer(s)
Comment "New entry."