MITE0000418
MITE0000419
MITE0000420
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Reaction Information

Number of Known Reactions: 2

Reaction 1
reaction SMARTS
Description Chlorination of ring B of the aglycone intermediate
Tailoring
  • Halogenation
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • In vitro assay
References
Example 1
Metadata
  • Description: C-8 chlorination of the aminocoumarin moiety
  • Intermediate: True
Substrate 1 substrate SMILES
Product 1 product SMILES
Reaction 2
reaction SMARTS
Description Bromination of ring B of the aglycone intermediate
Tailoring
  • Halogenation
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • In vitro assay
References
Example 1
Metadata
  • Description: C-8 bromination of the aminocoumarin moiety
  • Intermediate: True
Substrate 1 substrate SMILES
Product 1 product SMILES

Enzyme Information

Accession IDMITE0000419
Entry StatusStatus: active
Enzyme NameCloHal
Enzyme DescriptionFlavin-dependent halogenase
Database References
Cofactors
  • Organic
    • FAD
References
CommentHalogenation of the coupled aglycone containing ring A and B in the clorobiocin pathway. Bromine has higher affinity to CloHal but yields less bromobiocin due to lower turnover rate. Iodination and fluorination not possible
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2026-07-13)

Contributor(s)
Reviewer(s)
Comment "New entry."