MITE0000119
MITE0000120
MITE0000121
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Reaction Information

Number of Known Reactions: 2

Reaction 1
reaction SMARTS
Description Catalyzes ring contraction of a nine-membered ring to an eight-membered ring.
Tailoring
  • Ring contraction
Database References No database crosslinks available
Evidence
  • In vitro assay
  • Knock-out studies
References
Example 1
Metadata
  • Description: Ring contraction of 5-hydroxy-deoxyscytalidin to deoxyzopfiellin
  • Intermediate: True
Substrate 1 substrate SMILES
Product 1 product SMILES
Reaction 2
reaction SMARTS
Description Hydroxylation of nine-membered central ring in preparation of ring contraction
Tailoring
  • Hydroxylation
Database References No database crosslinks available
Evidence
  • Heterologous expression
  • In vitro assay
  • Knock-out studies
References
Example 1
Metadata
  • Description: Hydroxylation of deoxyscytalidin (C5) to 5-hydroxy-deoxyscytalidin
  • Intermediate: True
Substrate 1 substrate SMILES
Product 1 product SMILES

Enzyme Information

Accession IDMITE0000120
Entry StatusStatus: active
Enzyme NameZopL9/ZopK
Enzyme DescriptionAlpha-ketoglutarate dependent dioxygenase
Database References
Cofactors
  • Organic
    • a-Ketoglutaric acid
  • Inorganic
    • Fe
References
CommentαKG dependent enzyme that catalyzes oxidative ring contraction in the zopfiellin biosynthetic pathway. Previously referenced retired MIBiG ID BGC0002224.
Enzyme Visualization (AlphaFold-predicted)

Version 1 (2024-07-30)

Contributor(s)
  • Anonymous MITE contributor
Reviewer(s)
Comment "Initial entry. Reviewer fixed reactionSMARTS:reactionSMARTS. Reviewer fixed reactions:substrate. Reviewer fixed reactions:products. Reviewer 2 fixed incorrect protein databaseIDs (pointed to ZopA instead); fixed reaction SMARTS numbering"

Version 2 (2025-08-13)

Contributor(s)
  • Anonymous MITE contributor
Reviewer(s)
Comment "Fixed enzyme name to be more computer readable."

Version 3 (2026-04-13)

Contributor(s)
Reviewer(s)
Comment "Added additional reference and evidence qualifier (heterologous expression). Reviewer 2 fixed retired uniprot entry and removed retired MIBIG ID."

Auxiliary Enzyme #1

Enzyme Name ZopL2/ZopQ
Description Isochorismatase-like enzyme
Database References