{
    "accession": "MITE0000466",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-08",
            "contributors": [
                "0000-0003-2971-646X"
            ],
            "reviewers": [
                "0000-0003-3290-1017"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "SxtT",
        "description": "Rieske oxygenase",
        "references": [
            "doi:10.1021/jacs.8b08901"
        ],
        "databaseIds": {
            "uniprot": "C3RVQ0",
            "genpept": "ACG63840.1"
        },
        "cofactors": {
            "inorganic": [
                "FeS"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation",
                "Monooxygenation",
                "Oxidation"
            ],
            "description": "Hydroxylation",
            "reactionSMARTS": "[#7:1]12/[#6@@:2]3(-[#7:10]-[#6:11](=[#7:14])-[#7:12]-[#6@@:3]-3/[#6:4](-[#6:15]-[#8:16])-[#7:5]-[#6:6]-1=[#7:13])-[#6@@:7](/[#8:17])-[#6:8]-[#6:9]-2>>[#7:1]12/[#6@@:2]3(-[#7:10]-[#6:11](=[#7:14])-[#7:12]-[#6@@:3]-3/[#6:4](-[#6:15]-[#8:16])-[#7:5]-[#6:6]-1=[#7:13])-[#6@@:7](-[#8:18])(/[#8:17])-[#6:8]-[#6:9]-2",
            "reactions": [
                {
                    "substrate": "N=C1N[C@@H]2C(CO)NC(=N)N3CC[C@H](O)[C@@]23N1",
                    "products": [
                        "N=C1N[C@@H]2C(CO)NC(=N)N3CCC(O)(O)[C@@]23N1"
                    ],
                    "isIntermediate": true
                },
                {
                    "substrate": "N=C1N[C@@H]2C(COC(N)=O)NC(=N)N3CC[C@H](O)[C@@]23N1",
                    "products": [
                        "N=C1N[C@@H]2C(COC(N)=O)NC(=N)N3CCC(O)(O)[C@@]23N1"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1021/jacs.8b08901"
                ]
            }
        }
    ]
}