{
    "accession": "MITE0000463",
    "status": "active",
    "comment": "Catalyzes chlorination of tryptophan at C7 position to yield 7-chloro-L-tryptophan in pyrrolnitrin biosynthesis",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-06",
            "contributors": [
                "0000-0001-9487-1525"
            ],
            "reviewers": [
                "0000-0002-5203-4662",
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 1 added EC number. Reviewer 2 fixed crossref to MIBiG."
        }
    ],
    "enzyme": {
        "name": "PrnA",
        "description": "Tryptophan 7-halogenase",
        "references": [
            "doi:10.1126/science.1116510"
        ],
        "databaseIds": {
            "uniprot": "A8W2Z0",
            "genpept": "ABW69174.1",
            "mibig": "BGC0000924"
        },
        "cofactors": {
            "organic": [
                "FAD"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Halogenation"
            ],
            "description": "Chlorination of tryptophan at C7 position to yield 7-chloro-L-tryptophan in pyrrolnitrin biosynthesis",
            "reactionSMARTS": "[#6:1]1:[#6:6]2:[#6:9](-[#6:10]-[#6@@:11](\\[#7;+:14])-[#6:12](-[#8;-:13])=[#8:15]):[#6:8]:[#7:7]:[#6:5]:2:[#6:4]:[#6:3]:[#6:2]:1>>[#6:1]1:[#6:6]2:[#6:9](-[#6:10]-[#6@@:11](\\[#7;+:14])-[#6:12](-[#8;-:13])=[#8:15]):[#6:8]:[#7:7]:[#6:5]:2:[#6:4](-[Cl]):[#6:3]:[#6:2]:1",
            "reactions": [
                {
                    "substrate": "[NH3+][C@H](Cc1c[nH]c2ccccc12)C(=O)[O-]",
                    "products": [
                        "[NH3+][C@H](Cc1c[nH]c2c(Cl)cccc12)C(=O)[O-]"
                    ],
                    "isIntermediate": true,
                    "description": "Chlorination of tryptophan at C7 position to yield 7-chloro-L-tryptophan in pyrrolnitrin biosynthesis"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1126/science.1116510"
                ]
            },
            "databaseIds": {
                "ec": "1.14.19.9"
            }
        }
    ]
}