{
    "accession": "MITE0000459",
    "status": "active",
    "comment": "Catalyzes hydrozylation of m-hydroxybenzyl alcohol to gentisyl alcohol",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-06",
            "contributors": [
                "0009-0006-4188-3294"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "Fixed rxn SMARTS, added MIBiG reference, cofactor, evidence qualifier, textual additions."
        }
    ],
    "enzyme": {
        "name": "PatI",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1111/1462-2920.14542"
        ],
        "databaseIds": {
            "uniprot": "A0A075TMP8",
            "genpept": "AIG62145.1",
            "mibig": "BGC0000120"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "Hydroxylation of m-hydroxybenzyl alcohol to yield gentysil alcohol, step 4 of patulin biosynthesis",
            "reactionSMARTS": "[#8:1]-[#6:2]-[#6:3]1:[#6:9]:[#6:7](-[#8:8]):[#6:6]:[#6:5]:[#6:4]:1>>[#8:1]-[#6:2]-[#6:3]1:[#6:4](-[#8]):[#6:5]:[#6:6]:[#6:7](-[#8:8]):[#6:9]:1",
            "reactions": [
                {
                    "substrate": "OCc1cccc(O)c1",
                    "products": [
                        "OCc1cc(O)ccc1O"
                    ],
                    "isIntermediate": true,
                    "description": "Hydroxylation of m-hydroxybenzyl alcohol to gentysil alcohol"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1111/1462-2920.14542"
                ]
            }
        }
    ]
}