{
    "accession": "MITE0000458",
    "status": "active",
    "comment": "Thiocarboxylic acid S-methyltransferase",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-06",
            "contributors": [
                "0000-0002-3947-4444"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "New entry. Reviewer 1 redrew correct rxn SMARTS, redrew SMILES, added non-native rxn, textual additions, added MIBiG reference."
        }
    ],
    "enzyme": {
        "name": "YtkW",
        "description": "SAM-dependent methyltransferase",
        "references": [
            "doi:10.1002/anie.202522109"
        ],
        "databaseIds": {
            "uniprot": "I3NN71",
            "genpept": "ADZ13559.1",
            "mibig": "BGC0000466"
        },
        "cofactors": {
            "organic": [
                "SAM"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "S-methylation of thiocarboxylic acid moiety in the biosynthetic pathway of yatakemycin.",
            "reactionSMARTS": "[#6:1]1:[#6:12](-[#8:13]):[#6:11]:[#6:10]:[#6:3]2:[#6:4]:[#6:5](-[#6:7](=[#8:9])-[#16:8]):[#7:6]:[#6:2]:1:2>>[#6:1]1:[#6:12](-[#8:13]):[#6:11]:[#6:10]:[#6:3]2:[#6:4]:[#6:5](-[#6:7](=[#8:9])-[#16:8]-[#6]):[#7:6]:[#6:2]:1:2",
            "reactions": [
                {
                    "substrate": "COc1c(O)ccc2cc(C(=O)S)[nH]c12",
                    "products": [
                        "COc1c(O)ccc2cc(C(=O)SC)[nH]c12"
                    ],
                    "isIntermediate": true,
                    "description": "S-methylation on thiocarboxylic acid moiety of subunit A of yatakemycin."
                },
                {
                    "substrate": "COc1cc2cc(C(=O)S)[nH]c2cc1O",
                    "products": [
                        "COc1cc2cc(C(=O)SC)[nH]c2cc1O"
                    ],
                    "isIntermediate": true,
                    "description": "S-methylation on thiocarboxylic acid moiety of a non-native substrate analogue"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1002/anie.202522109"
                ]
            },
            "databaseIds": {
                "ec": "2.1.1"
            }
        }
    ]
}