{
    "accession": "MITE0000452",
    "status": "active",
    "comment": "PrnD (aminopyrrolnitrin oxygenase) catalyzes the final N-oxygenation step in pyrrolnitrin biosynthesis, converting aminopyrrolnitrin into pyrrolnitrin by oxidation of an arylamine to an arylnitro group",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-14",
            "contributors": [
                "0009-0005-2489-2079"
            ],
            "reviewers": [
                "0000-0002-4540-6501"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "PrnD",
        "description": "Rieske N-oxygenase",
        "references": [
            "doi:10.1074/jbc.M505334200",
            "doi:10.1111/j.1574-6941.2009.00792.x."
        ],
        "databaseIds": {
            "uniprot": "A8W2Z3",
            "genpept": "ABW69177.1",
            "mibig": "BGC0000924"
        },
        "cofactors": {
            "organic": [
                "Flavin mononucleotide",
                "NADP"
            ],
            "inorganic": [
                "Fe",
                "FeS"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Monooxygenation",
                "Oxidation"
            ],
            "description": "Oxidation of the arylamine group of aminopyrrolnitrin to the corresponding arylnitro group in pyrrolnitrin.",
            "reactionSMARTS": "[#6:1]1:[#6:5]:[#6:6](-[Cl:9]):[#6:4](-[#7]):[#6:2](-[#6:7]2:[#6:11]:[#7:10]:[#6:12]:[#6:13]:2-[Cl:14]):[#6:3]:1>>[#6:6]1(-[Cl:9]):[#6:4](-[#7;+](-[#8;-])=[#8]):[#6:2](-[#6:7]2:[#6:11]:[#7:10]:[#6:12]:[#6:13]:2-[Cl:14]):[#6:3]:[#6:1]:[#6:5]:1",
            "reactions": [
                {
                    "substrate": "Nc1c(Cl)cccc1-c1c[nH]cc1Cl",
                    "products": [
                        "O=[N+]([O-])c1c(Cl)cccc1-c1c[nH]cc1Cl"
                    ],
                    "isIntermediate": false,
                    "description": "Oxidation of the arylamine group of aminopyrrolnitrin to the corresponding arylnitro group in pyrrolnitrin"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay",
                    "Site-directed mutagenesis"
                ],
                "references": [
                    "doi:10.1074/jbc.M505334200"
                ]
            },
            "databaseIds": {
                "rhea": "46136"
            }
        }
    ]
}