{
    "accession": "MITE0000447",
    "status": "active",
    "comment": "NanP a putative monooxygenase, which hydroxylates at C-30 at the  ACP-bound nanchangmycin aglycone",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-28",
            "contributors": [
                "0009-0008-8086-5325"
            ],
            "reviewers": [
                "0000-0002-4540-6501"
            ],
            "comment": "Changed Description and reference 2 added"
        }
    ],
    "enzyme": {
        "name": "NanP",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1016/S1074-5521(03)00092-9",
            "doi:10.1007/s00203-011-0768-8"
        ],
        "databaseIds": {
            "uniprot": "UPI000019B38C",
            "genpept": "AAP42875.1",
            "mibig": "BGC0000105"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation",
                "Oxidation"
            ],
            "description": "NanP is involved in the oxidation of the methyl group at C-30 to generate ACP-bound nanchangmycin aglycone",
            "reactionSMARTS": "[#6:1]-[#6:2](-[#6:42](-[#6:44](-[#6:46]-[#6:47](-[#6:49](-[*])=[#8:50])-[#6:48])-[#6:45])=[#8:43])-[#6:3]-[#6:4](-[#6:6]1-[#6:40](-[#6:41])-[#6:38](-[#8:39])-[#6:37]-[#6:8]2(-[#8:36]-[#6:11](-[#6:13]3-[#8:35]-[#6:19]4(-[#6:33](-[#6:34])-[#6:32]-[#6:21](-[#6:22]5-[#6:30](-[#6:31])-[#6:29]-[#6:27](-[#6:28])-[#6:24](-[#8:26])(-[#6:25])-[#8:23]-5)-[#8:20]-4)-[#6:17](-[#6:18])-[#6:15](-[#8:16])-[#6:14]-3)(-[#6:12])-[#6:10]-[#6:9]-2)-[#8:7]-1)-[#6:5]>>[#6:49](-[*])(-[#6:47](-[#6:48])-[#6:46]-[#6:44](-[#6:45])-[#6:42](=[#8:43])-[#6:2](-[#6:3]-[#6:4](-[#6:6]1-[#6:40](-[#6:41])-[#6:38](-[#8:39])-[#6:37]-[#6:8]2(-[#8:36]-[#6:11](-[#6:13]3-[#8:35]-[#6:19]4(-[#6:33](-[#6:34])-[#6:32]-[#6:21](-[#6:22]5-[#6:30](-[#6:31])-[#6:29]-[#6:27](-[#6:28])-[#6:24](-[#6:25]-[#8])(-[#8:26])-[#8:23]-5)-[#8:20]-4)-[#6:17](-[#6:18])-[#6:15](-[#8:16])-[#6:14]-3)(-[#6:12])-[#6:10]-[#6:9]-2)-[#8:7]-1)-[#6:5])-[#6:1])=[#8:50]",
            "reactions": [
                {
                    "substrate": "*C(=O)C(C)CC(C)C(=O)C(C)CC(C)C1OC2(CCC(C)(C3CC(O)C(C)C4(OC(C5OC(C)(O)C(C)CC5C)CC4C)O3)O2)CC(O)C1C",
                    "products": [
                        "*C(=O)C(C)CC(C)C(=O)C(C)CC(C)C1OC2(CCC(C)(C3CC(O)C(C)C4(OC(C5OC(O)(CO)C(C)CC5C)CC4C)O3)O2)CC(O)C1C"
                    ],
                    "isIntermediate": true,
                    "description": "NanP a putative monooxygenase, which hydroxylates at C-30 at the  ACP-bound nanchangmycin aglycone"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Inference from genomic data and chemical structure"
                ],
                "references": [
                    "doi:10.1016/S1074-5521(03)00092-9",
                    "doi:10.1016/j.chembiol.2008.04.006"
                ]
            }
        }
    ]
}