{
    "accession": "MITE0000444",
    "status": "active",
    "comment": "Catalyzes N-hydroxylation of nanangelenin C.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-29",
            "contributors": [
                "0000-0001-9260-4650"
            ],
            "reviewers": [
                "0000-0002-4540-6501"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "NanF",
        "description": "FAD-dependent oxidoreductase",
        "references": [
            "doi:10.1021/jacs.0c01605"
        ],
        "databaseIds": {
            "uniprot": "A0A6G9KH61",
            "genpept": "QIQ51367.1"
        },
        "cofactors": {
            "organic": [
                "FAD"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation",
                "Oxidation"
            ],
            "description": "N-hydroxylation of nanangelenin C",
            "reactionSMARTS": "[#6:1]12:[#6:28]:[#6:27]:[#6:26]:[#6:25]:[#6:24]:1-[#6:22](=[#8:23])-[#6:21]-[#6@:5](-[#7:6]-[#6:7](-[#6:9]1:[#6:14](-[#7:15]-[#6:16](-[#6:19]=[#6:20])(-[#6:18])-[#6:17]):[#6:13]:[#6:12]:[#6:11]:[#6:10]:1)=[#8:8])-[#6:3](=[#8:4])-[#7:2]-2>>[#6:1]12:[#6:28]:[#6:27]:[#6:26]:[#6:25]:[#6:24]:1-[#6:22](=[#8:23])-[#6:21]-[#6@:5](-[#7:6]-[#6:7](-[#6:9]1:[#6:14](-[#7:15](-[#8])-[#6:16](-[#6:19]=[#6:20])(-[#6:18])-[#6:17]):[#6:13]:[#6:12]:[#6:11]:[#6:10]:1)=[#8:8])-[#6:3](=[#8:4])-[#7:2]-2",
            "reactions": [
                {
                    "substrate": "C=CC(C)(C)Nc1ccccc1C(=O)N[C@H]1CC(=O)c2ccccc2NC1=O",
                    "products": [
                        "C=CC(C)(C)N(O)c1ccccc1C(=O)N[C@H]1CC(=O)c2ccccc2NC1=O"
                    ],
                    "isIntermediate": true,
                    "description": "N-hydroxylation of nanangelenin C"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1021/jacs.0c01605"
                ]
            }
        }
    ]
}