{
    "accession": "MITE0000437",
    "status": "active",
    "comment": "Hydroxylation",
    "changelog": [
        {
            "version": "1",
            "date": "2026-06-14",
            "contributors": [
                "0009-0008-8086-5325"
            ],
            "reviewers": [
                "0000-0002-8323-5416"
            ],
            "comment": "New entry. Reviewer 1 added NADP as a cofactor"
        }
    ],
    "enzyme": {
        "name": "BcmD",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1038/s41598-019-56747-7",
            "doi:10.1021/acs.biochem.7b00943"
        ],
        "databaseIds": {
            "uniprot": "UPI000C089C81",
            "genpept": "PPT15946.1",
            "mibig": "BGC0001468"
        },
        "cofactors": {
            "organic": [
                "Heme",
                "NADP"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "BcmD catalyzes hydroxylation to form intermediate product dihydrobicyclomycin in the biosynthesis of bicyclomycin.",
            "reactionSMARTS": "[#6:2](-[#6@:9]12\\[#8:20]-[#6:19]-[#6:18]-[#6:17](-[#6:21])-[#6:13](-[#7:14]-[#6:15]-1=[#8:16])-[#6:11](=[#8:12])-[#7:10]-2)(-[#6@:4](-[#6:7]-[#8:8])(\\[#8:6])-[#6:5])-[#8:3]>>[#6:2](-[#6@:9]12\\[#8:20]-[#6:19]-[#6:18]-[#6:17](-[#6:21])-[#6@@:13](-[#7:14]-[#6:15]-1=[#8:16])(/[#8:3])-[#6:11](=[#8:12])-[#7:10]-2)(-[#6@:4](-[#6:7]-[#8:8])(\\[#8:6])-[#6:5])-[#8:3]",
            "reactions": [
                {
                    "substrate": "CC1CCO[C@]2(C(O)[C@@](C)(O)CO)NC(=O)C1NC2=O",
                    "products": [
                        "CC1CCO[C@]2(C(O)[C@@](C)(O)CO)NC(=O)[C@@]1(O)NC2=O"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1038/s41598-019-56747-7",
                    "doi:10.1021/acs.biochem.7b00943"
                ]
            }
        }
    ]
}