{
    "accession": "MITE0000436",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2026-06-14",
            "contributors": [
                "0009-0008-8086-5325"
            ],
            "reviewers": [
                "0000-0002-8323-5416"
            ],
            "comment": "New entry. Reviewer 1 added 2-OG and Fe as cofactors."
        }
    ],
    "enzyme": {
        "name": "BcmF",
        "description": "2OG-Fe(II) oxygenase",
        "references": [
            "doi:10.1038/s41598-019-56747-7",
            "doi:10.1021/acs.biochem.7b00943"
        ],
        "databaseIds": {
            "uniprot": "UPI000C07B921",
            "genpept": "PPT15945.1",
            "mibig": "BGC0001468"
        },
        "cofactors": {
            "organic": [
                "a-Ketoglutaric acid"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Dehydrogenation"
            ],
            "description": "BcmF performs dehydrogenation of the isoleucyl side chain to produce the final product bicyclomycin",
            "reactionSMARTS": "[#6:1](-[#8:21])(-[#6@@:16](-[#6:20])(/[#8:19])-[#6:17]-[#8:18])\\[#6@@:2]12-[#7:15]-[#6:13](=[#8:14])-[#6@@:8](\\[#8:12])(-[#7:9]-[#6:10]-1=[#8:11])-[#6:6](-[#6])-[#6:5]-[#6:4]-[#8:3]-2>>[#6:1](-[#8:21])(-[#6@@:16](-[#6:20])(/[#8:19])-[#6:17]-[#8:18])\\[#6@@:2]12-[#7:15]-[#6:13](=[#8:14])-[#6@@:8](\\[#8:12])(-[#7:9]-[#6:10]-1=[#8:11])-[#6:6](=[#6:1])-[#6:5]-[#6:4]-[#8:3]-2",
            "reactions": [
                {
                    "substrate": "CC1CCO[C@]2(C(O)[C@@](C)(O)CO)NC(=O)[C@@]1(O)NC2=O",
                    "products": [
                        "C=C1CCO[C@]2(C(O)[C@@](C)(O)CO)NC(=O)[C@@]1(O)NC2=O"
                    ],
                    "isIntermediate": false
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1038/s41598-019-56747-7"
                ]
            }
        }
    ]
}