{
    "accession": "MITE0000434",
    "status": "active",
    "comment": "A cytochrome P450 monooxygenase, AmyB is responsible for the atropospecific formation of one carbon-carbon and two carbon-nitrogen bonds on precursor peptide",
    "changelog": [
        {
            "version": "1",
            "date": "2026-06-22",
            "contributors": [
                "0009-0008-8086-5325"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 1 added cofactors."
        }
    ],
    "enzyme": {
        "name": "AmyB",
        "description": "Cytochrome P40",
        "references": [
            "doi:10.1002/anie.202208361"
        ],
        "databaseIds": {
            "uniprot": "UPI000898B31C",
            "genpept": "SDY48146.1",
            "mibig": "BGC0002751"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Biaryl bond formation",
                "Other"
            ],
            "description": "AmyB introduces atropospecific one carbon-carbon and two carbon-nitrogen bonds",
            "reactionSMARTS": "[#6:1]-[#6:2]-[#6:3](-[#6:5](-[#6:33](-[#7:35]-[#6:36](-[#6:47](-[#7:49]-[#6:50](-[#6:61](-[#8:63])=[#8:62])-[#6:51]-[#6:52]1:[#6:60]2:[#6:55](:[#6:56]:[#6:57]:[#6:58]:[#6:59]:2):[#7:54]:[#6:53]:1)=[#8:48])-[#6:37]-[#6:38]1:[#6:46]2:[#6:41](:[#6:42]:[#6:43]:[#6:44]:[#6:45]:2):[#7:40]:[#6:39]:1)=[#8:34])-[#7:6]-[#6:7](-[#6:9](-[#7:18]-[#6:19](-[#6:21](-[#6:23]-[#6:24]1:[#6:32]2:[#6:27](:[#6:28]:[#6:29]:[#6:30]:[#6:31]:2):[#7:26]:[#6:25]:1)-[#7:22])=[#8:20])-[#6:10]-[#6:11]1:[#6:17]:[#6:16]:[#6:14](-[#8:15]):[#6:13]:[#6:12]:1)=[#8:8])-[#6:4]>>[#6:1]-[#6:2]-[#6:3](-[#6:5]1-[#6:33](=[#8:34])-[#7:35]2-[#6:39]3-[#7:40]-[#6:41]4:[#6:46](-[#6:38]-3(-[#6:37]-[#6:36]-2-[#6:47](-[#7:49]-[#6:50](-[#6:61](-[#8:63])=[#8:62])-[#6:51]-[#6:52]2:[#6:60]3:[#6:55](:[#6:56]:[#6:57]:[#6:58]:[#6:59]:3):[#7:54]:[#6:53]:2)=[#8:48])-[#6:30]2:[#6:29]:[#6:28]:[#6:27]3:[#7:26]5:[#6:25]:[#6:24](-[#6:23]-[#6:21](-[#6:19](-[#7:18]-[#6:9](-[#6:10]-[#6:11]6:[#6:12]:[#6:13]-5:[#6:14](-[#8:15]):[#6:16]:[#6:17]:6)-[#6:7](=[#8:8])-[#7:6]-1)=[#8:20])-[#7:22]):[#6:32]:3:[#6:31]:2):[#6:45]:[#6:44]:[#6:43]:[#6:42]:4)-[#6:4]",
            "reactions": [
                {
                    "substrate": "CCC(C)C(NC(=O)C(Cc1ccc(O)cc1)NC(=O)C(N)Cc1c[nH]c2ccccc12)C(=O)NC(Cc1c[nH]c2ccccc12)C(=O)NC(Cc1c[nH]c2ccccc12)C(=O)O",
                    "products": [
                        "CCC(C)C1NC(=O)C2Cc3ccc(O)c(c3)-n3cc(c4cc(ccc43)C34CC(C(=O)NC(Cc5c[nH]c6ccccc56)C(=O)O)N(C1=O)C3Nc1ccccc14)CC(N)C(=O)N2"
                    ],
                    "isIntermediate": true,
                    "description": "AmyB introduces one C-C and two C-N bonds on the precursor peptide followed by maturation by protease yielding hexapeptide amyxirubin A (AWYIWW) and pentapeptide amyxirubin B (WYIWW)"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1002/anie.202208361"
                ]
            }
        }
    ]
}