{
    "accession": "MITE0000431",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2026-06-25",
            "contributors": [
                "0009-0008-8086-5325"
            ],
            "reviewers": [
                "0000-0001-5701-0590"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "ThdD",
        "description": "Amidotransferase",
        "references": [
            "doi:10.1002/cbic.201400016",
            "doi:10.1002/cbic.201500670"
        ],
        "databaseIds": {
            "uniprot": "UPI0002B76286",
            "genpept": "ANW12109.1",
            "mibig": "BGC0001374"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Amination"
            ],
            "description": "ThdD (homologue to ThnA) catalyzes the amidation of the carboxylic acid to complete thienodolin biosynthesis",
            "reactionSMARTS": "[#8:1]=[#6:2](-[#6:4]1:[#16:16]:[#6:7]2:[#7:8]:[#6:9]3:[#6:15](:[#6:6]:2:[#6:5]:1):[#6:14]:[#6:13]:[#6:11](-[Cl:12]):[#6:10]:3)-[#8]>>[#16:16]1:[#6:7]2:[#6:6](:[#6:5]:[#6:4]:1-[#6:2](=[#8:1])-[#7:8]):[#6:15]1:[#6:14]:[#6:13]:[#6:11](:[#6:10]:[#6:9]:1:[#7:8]:2)-[Cl:12]",
            "reactions": [
                {
                    "substrate": "O=C(O)c1cc2c([nH]c3cc(Cl)ccc32)s1",
                    "products": [
                        "NC(=O)c1cc2c([nH]c3cc(Cl)ccc32)s1"
                    ],
                    "isIntermediate": false,
                    "description": "ThdD as an amidotransferase catalyzes the conversion of the carboxylic acid group of 6-chlorothieno[2,3-b]indole-2-carboxylic acid to the amido group of thienodolin"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1002/cbic.201400016"
                ]
            }
        }
    ]
}