{
    "accession": "MITE0000427",
    "status": "active",
    "comment": "Rdc2 is a flavin-dependent halogenase involved in the biosynthesis of the fungal metabolite radicicol.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-07-01",
            "contributors": [
                "0009-0008-8086-5325"
            ],
            "reviewers": [
                "0000-0002-8323-5416"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "Rdc2",
        "description": "Halogenase",
        "references": [
            "doi:10.1128/AEM.00478-08",
            "doi:10.1074/jbc.M110.183574",
            "doi:10.1016/j.bmcl.2012.12.038"
        ],
        "databaseIds": {
            "uniprot": "UPI00017691F7",
            "genpept": "ACD39771.1",
            "mibig": "BGC0000134"
        },
        "cofactors": {
            "organic": [
                "FAD"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Halogenation"
            ],
            "description": "Rdc2 catalyzes the regioselective chlorination of a late-stage intermediate (monocillin II to pochonin D) during radicicol biosynthesis",
            "reactionSMARTS": "[#6:1]-[#6:2]1-[#8:17]-[#6:15](=[#8:16])-[#6:14]2:[#6:18](:[#6:20]:[#6:21](:[#6:23]:[#6:13]:2-[#6:12]-[#6:10](=[#8:11])-[#6:9]=[#6:8]-[#6:7]-[#6:6]-[#6:5]=[#6:4]-[#6:3]-1)-[#8:22])-[#8:19]>>[#6:1]-[#6:2]1-[#8:17]-[#6:15](=[#8:16])-[#6:14]2:[#6:18](:[#6:20]:[#6:21](:[#6:23](-[Cl]):[#6:13]:2-[#6:12]-[#6:10](=[#8:11])-[#6:9]=[#6:8]-[#6:7]-[#6:6]-[#6:5]=[#6:4]-[#6:3]-1)-[#8:22])-[#8:19]",
            "reactions": [
                {
                    "substrate": "CC1CC=CCCC=CC(=O)Cc2cc(O)cc(O)c2C(=O)O1",
                    "products": [
                        "CC1CC=CCCC=CC(=O)Cc2c(Cl)c(O)cc(O)c2C(=O)O1"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1128/AEM.00478-08",
                    "doi:10.1074/jbc.M110.183574"
                ]
            }
        },
        {
            "tailoring": [
                "Halogenation"
            ],
            "description": "Rdc2 can also halogenate hydroxyisoquinoline to produce chlorinated hydroxyisoquinolines",
            "reactionSMARTS": "[#6:1]1:[#6:10]:[#6:9]2:[#6:4](:[#6:5]:[#7:6]:[#6:7]:[#6:8]:2-[#8:11]):[#6:3]:[#6:2]:1>>[#6:1]1:[#6:10]:[#6:9]2:[#6:4](:[#6:5]:[#7:6]:[#6:7](-[Cl]):[#6:8]:2-[#8:11]):[#6:3]:[#6:2]:1",
            "reactions": [
                {
                    "substrate": "Oc1cncc2ccccc12",
                    "products": [
                        "Oc1c(Cl)ncc2ccccc12"
                    ],
                    "isIntermediate": false
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1016/j.bmcl.2012.12.038"
                ]
            }
        }
    ]
}