{
    "accession": "MITE0000424",
    "status": "active",
    "comment": "DolJ performs the terminal oxidative decarboxylation in the Dolastatin 10 biosynthesis",
    "changelog": [
        {
            "version": "1",
            "date": "2026-07-05",
            "contributors": [
                "0009-0003-3080-8430"
            ],
            "reviewers": [
                "0000-0002-8323-5416"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "DolJ",
        "description": "Non-Heme Diiron Monooxygenase",
        "references": [
            "doi:10.1021/acs.orglett.3c04083"
        ],
        "databaseIds": {
            "uniprot": "UPI003369BCD6",
            "genpept": "WZC35280.1",
            "mibig": "BGC0002886"
        },
        "cofactors": {
            "organic": [
                "Ascorbic acid"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Decarboxylation"
            ],
            "description": "DolJ catalyzes oxidative decarboxylation to form the terminal thiazole moiety of Dolastatin 10",
            "reactionSMARTS": "[#6:1]-[#6:2]-[#6@@:3](-[#6@:5](/[#7:38](-[#6:40](\\[#6@:42](-[#6:53](-[#6:55])-[#6:54])-[#7:43]-[#6:44](/[#6@@:46](-[#7:50](-[#6:52])-[#6:51])-[#6:47](-[#6:49])-[#6:48])=[#8:45])=[#8:41])-[#6:39])-[#6@:6](-[#8:36]-[#6:37])/[#6:7]-[#6:8](-[#7:10]1-[#6@:14](-[#6@@:15](-[#6@:18](-[#6:20](-[#7:22]/[#6@:23](-[#6:31]2:[#16:35]:[#6:34]:[#6@@:33](\\[#6](=[#8])-[#8]):[#7:32]:2)-[#6:24]-[#6:25]2:[#6:30]:[#6:29]:[#6:28]:[#6:27]:[#6:26]:2)=[#8:21])/[#6:19])/[#8:16]-[#6:17])/[#6:13]-[#6:12]-[#6:11]-1)=[#8:9])\\[#6:4]>>[#6:1]-[#6:2]-[#6@@:3](-[#6@:5](/[#7:38](-[#6:40](\\[#6@:42](-[#6:53](-[#6:54])-[#6:55])-[#7:43]-[#6:44](/[#6@@:46](-[#7:50](-[#6:52])-[#6:51])-[#6:47](-[#6:48])-[#6:49])=[#8:45])=[#8:41])-[#6:39])-[#6@:6](-[#8:36]-[#6:37])/[#6:7]-[#6:8](-[#7:10]1-[#6@:14](-[#6@@:15](-[#6@:18](-[#6:20](-[#7:22]/[#6@:23](-[#6:31]2:[#16:35]:[#6:34]:[#6:33]:[#7:32]:2)-[#6:24]-[#6:25]2:[#6:26]:[#6:27]:[#6:28]:[#6:29]:[#6:30]:2)=[#8:21])/[#6:19])/[#8:16]-[#6:17])/[#6:13]-[#6:12]-[#6:11]-1)=[#8:9])\\[#6:4]",
            "reactions": [
                {
                    "substrate": "CC[C@H](C)[C@@H]([C@@H](CC(=O)N1CCC[C@H]1[C@H](OC)[C@@H](C)C(=O)N[C@@H](Cc1ccccc1)c1nc(C(=O)O)cs1)OC)N(C)C(=O)[C@@H](NC(=O)[C@H](C(C)C)N(C)C)C(C)C",
                    "products": [
                        "CC[C@H](C)[C@@H]([C@@H](CC(=O)N1CCC[C@H]1[C@H](OC)[C@@H](C)C(=O)N[C@@H](Cc1ccccc1)c1nccs1)OC)N(C)C(=O)[C@@H](NC(=O)[C@H](C(C)C)N(C)C)C(C)C"
                    ],
                    "isIntermediate": false
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1021/acs.orglett.3c04083"
                ]
            }
        }
    ]
}