{
    "accession": "MITE0000423",
    "status": "active",
    "comment": "BarH performs the terminal oxidative decarboxylation to form fully oxidized, conjugated thiazole ring in the biosynthesis of barbamide",
    "changelog": [
        {
            "version": "1",
            "date": "2026-07-05",
            "contributors": [
                "0009-0008-8086-5325"
            ],
            "reviewers": [
                "0000-0002-8323-5416"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "BarH",
        "description": "Non-Heme Diiron Monooxygenase",
        "references": [
            "doi:10.1021/acs.orglett.3c04083",
            "doi:10.1016/S0378-1119(02)00860-0"
        ],
        "databaseIds": {
            "uniprot": "UPI00000C04FF",
            "genpept": "AAN32982.1"
        },
        "cofactors": {
            "organic": [
                "Ascorbic acid"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Decarboxylation",
                "Dehydrogenation"
            ],
            "description": "BarH catalyzes the terminal oxidative decarboxylation and final dehydrogenation that generates a thiazole ring from a thiazoline intermediate during the biosynthesis of barbamide",
            "reactionSMARTS": "[#6:1]-[#6:2](-[#6:25](-[Cl:28])(-[Cl:27])-[Cl:26])-[#6:3]-[#6:4](-[#8:23]-[#6:24])-[#6:5]-[#6:6](-[#7:8](-[#6:10](-[#6:18]1-[#16:22]-[#6:21]-[#6:20](-[#6](-[#8])=[#8])-[#7:19]=1)-[#6:11]-[#6:12]1:[#6:17]:[#6:16]:[#6:15]:[#6:14]:[#6:13]:1)-[#6:9])=[#8:7]>>[#6:1]-[#6:2](-[#6:25](-[Cl:28])(-[Cl:26])-[Cl:27])-[#6:3]-[#6:4](-[#8:23]-[#6:24])-[#6:5]-[#6:6](-[#7:8](-[#6:10](-[#6:18]1:[#16:22]:[#6:21]:[#6:20]:[#7:19]:1)-[#6:11]-[#6:12]1:[#6:17]:[#6:16]:[#6:15]:[#6:14]:[#6:13]:1)-[#6:9])=[#8:7]",
            "reactions": [
                {
                    "substrate": "COC(CC(=O)N(C)C(Cc1ccccc1)C1=NC(C(=O)O)CS1)CC(C)C(Cl)(Cl)Cl",
                    "products": [
                        "COC(CC(=O)N(C)C(Cc1ccccc1)c1nccs1)CC(C)C(Cl)(Cl)Cl"
                    ],
                    "isIntermediate": false
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1021/acs.orglett.3c04083"
                ]
            }
        }
    ]
}