{
    "accession": "MITE0000422",
    "status": "active",
    "comment": "LynB7 functions as a termination-stage oxidative decarboxylase that synthesizes the C-terminal thiazole ring of lyngbyapeptin B",
    "changelog": [
        {
            "version": "1",
            "date": "2026-07-05",
            "contributors": [
                "0009-0008-8086-5325"
            ],
            "reviewers": [
                "0000-0002-8323-5416"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "LynB7",
        "description": "Non-Heme Diiron Oxygenase",
        "references": [
            "doi:10.1021/acschembio.3c00011"
        ],
        "databaseIds": {
            "uniprot": "UPI00419E3F48",
            "genpept": "BBU25148.1"
        },
        "cofactors": {
            "organic": [
                "Ascorbic acid"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Decarboxylation",
                "Dehydrogenation"
            ],
            "description": "LynB7 catalyzes the oxidative decarboxylation of 2-thiazoline-4-carboxylic acid to fully conjugated 2-thiazole moiety in the lyngbyapeptin B biosynthesis",
            "reactionSMARTS": "[#6:1]/[#6@:2](-[#7:8]-[#6:9](/[#6@@:11](-[#7:21](-[#6:23](/[#6@@:25](-[#7:29](-[#6:31](\\[#6@@:33](-[#7:43](-[#6:45](-[#6:47]-[#6:48](-[#8:50]-[#6:51])-[#6:49])=[#8:46])-[#6:44])-[#6:34]-[#6:35]1:[#6:40]:[#6:39]:[#6:38](-[#8:41]-[#6:42]):[#6:37]:[#6:36]:1)=[#8:32])-[#6:30])-[#6:26](-[#6:28])-[#6:27])=[#8:24])-[#6:22])-[#6:12]-[#6:13]1:[#6:18]:[#6:17]:[#6:16](-[#8:19]-[#6:20]):[#6:15]:[#6:14]:1)=[#8:10])-[#6:3]1-[#16:7]-[#6:6]/[#6@@:5](-[#6](-[#8])=[#8])-[#7:4]=1>>[#6:1]/[#6@:2](-[#7:8]-[#6:9](/[#6@@:11](-[#7:21](-[#6:23](/[#6@@:25](-[#7:29](-[#6:31](\\[#6@@:33](-[#7:43](-[#6:45](-[#6:47]-[#6:48](-[#8:50]-[#6:51])-[#6:49])=[#8:46])-[#6:44])-[#6:34]-[#6:35]1:[#6:40]:[#6:39]:[#6:38](-[#8:41]-[#6:42]):[#6:37]:[#6:36]:1)=[#8:32])-[#6:30])-[#6:26](-[#6:27])-[#6:28])=[#8:24])-[#6:22])-[#6:12]-[#6:13]1:[#6:14]:[#6:15]:[#6:16](-[#8:19]-[#6:20]):[#6:17]:[#6:18]:1)=[#8:10])-[#6:3]1:[#16:7]:[#6:6]:[#6:5]:[#7:4]:1",
            "reactions": [
                {
                    "substrate": "COc1ccc(C[C@@H](C(=O)N(C)[C@H](C(=O)N(C)[C@@H](Cc2ccc(OC)cc2)C(=O)N[C@@H](C)C2=N[C@H](C(=O)O)CS2)C(C)C)N(C)C(=O)CC(C)OC)cc1",
                    "products": [
                        "COc1ccc(C[C@@H](C(=O)N(C)[C@H](C(=O)N(C)[C@@H](Cc2ccc(OC)cc2)C(=O)N[C@@H](C)c2nccs2)C(C)C)N(C)C(=O)CC(C)OC)cc1"
                    ],
                    "isIntermediate": false
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1021/acschembio.3c00011"
                ]
            }
        }
    ]
}