{
    "accession": "MITE0000420",
    "status": "active",
    "comment": "BytZ encodes a putative S-adenosyl-methionine (SAM)-dependent methyltransferase proposed to catalyze N-terminal methylation after the removal of the leader peptide to produce myxarylin",
    "changelog": [
        {
            "version": "1",
            "date": "2026-07-07",
            "contributors": [
                "0009-0008-8086-5325"
            ],
            "reviewers": [
                "0000-0001-5863-7248"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "BytZ",
        "description": "N-methyltransferase",
        "references": [
            "doi:10.3390/molecules26247483",
            "doi:10.1021/jacs.5c17257"
        ],
        "databaseIds": {
            "genpept": "UID85537.1",
            "mibig": "BGC0002631"
        },
        "cofactors": {
            "organic": [
                "SAM"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "BytZ catalyzes N-terminal methylation of the mature cyclized peptide.",
            "reactionSMARTS": "[#7:1]/[#6@@:2]1-[#6:30](=[#8:31])-[#7:29]-[#6@@:24](\\[#6:25]-[#6:26](-[#6:28])-[#6:27])-[#6:22](=[#8:23])-[#7:21]-[#6@:17](/[#6:18](-[#8:20])=[#8:19])-[#6:16]-[#6:15]2:[#7:11](:[#6:12]:[#7:13]:[#6:14]:2)-[#6:9]2:[#6:10]:[#6:4](:[#6:5]:[#6:6]:[#6:7]:2-[#8:8])-[#6:3]-1>>[#6]-[#7:1]/[#6@@:2]1-[#6:30](=[#8:31])-[#7:29]-[#6@@:24](\\[#6:25]-[#6:26](-[#6:28])-[#6:27])-[#6:22](=[#8:23])-[#7:21]-[#6@:17](/[#6:18](-[#8:20])=[#8:19])-[#6:16]-[#6:15]2:[#7:11](:[#6:12]:[#7:13]:[#6:14]:2)-[#6:9]2:[#6:10]:[#6:4](:[#6:5]:[#6:6]:[#6:7]:2-[#8:8])-[#6:3]-1",
            "reactions": [
                {
                    "substrate": "CC(C)C[C@@H]1NC(=O)[C@@H](N)Cc2ccc(O)c(c2)-n2cncc2C[C@@H](C(=O)O)NC1=O",
                    "products": [
                        "CN[C@H]1Cc2ccc(O)c(c2)-n2cncc2C[C@@H](C(=O)O)NC(=O)[C@H](CC(C)C)NC1=O"
                    ],
                    "isIntermediate": false
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "Inference from genomic data and chemical structure"
                ],
                "references": [
                    "doi:10.3390/molecules26247483",
                    "doi:10.1021/jacs.5c17257"
                ]
            }
        }
    ]
}