{
    "accession": "MITE0000418",
    "status": "active",
    "comment": "Single chlorination of carrierbound-S-isoleucine (L-Ile-S-KtzC) in the Kutzneride pathway. UniProt A8CF92 retired. Non Substrate: Allo-isoleucine",
    "changelog": [
        {
            "version": "1",
            "date": "2026-06-24",
            "contributors": [
                "0009-0000-8651-1669"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "KtzD",
        "description": "Nonheme halogenase",
        "references": [
            "doi:10.1021/ja8064667"
        ],
        "databaseIds": {
            "uniprot": "UPI00015D3A82",
            "genpept": "ABV56584.1",
            "mibig": "BGC0000378"
        },
        "cofactors": {
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Halogenation"
            ],
            "description": "Oxygen and carrierprotein KtzC dependent halogenation",
            "reactionSMARTS": "[#6:1]-[#6:2]-[#6:3](-[#6:5](-[#6:7](-[#16:9]-[*])=[#8:8])\\[#7:6])/[#6:4]>>[#6:1]-[#6:2]-[#6:3](-[#6:5](-[#6:7](-[#16:9]-[*])=[#8:8])\\[#7:6])/[#6:4]-[Cl]",
            "reactions": [
                {
                    "substrate": "*SC(=O)[C](N)[C](C)CC",
                    "products": [
                        "*SC(=O)C(N)C(CC)CCl"
                    ],
                    "isIntermediate": true,
                    "description": "Single chlorination of L-Isoleucine-S-KtzC"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1021/ja8064667"
                ]
            }
        }
    ]
}