{
    "accession": "MITE0000412",
    "status": "active",
    "comment": "Cytochrome P450 catalyzing the hydroxylation of peregrinol to form labd-13Z-ene-9,15,16-triol",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-13",
            "contributors": [
                "0000-0002-7239-6767"
            ],
            "reviewers": [
                "0000-0003-4819-2251"
            ],
            "comment": "New entry. Reviewer 1 fixed reaction SMARTS, added cofactor and evidence qualifier."
        }
    ],
    "enzyme": {
        "name": "VacCYP76BK",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1111/tpj.13822"
        ],
        "databaseIds": {
            "uniprot": "A0A2K9RG08",
            "genpept": "AUT77126.1"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation",
                "Monooxygenation"
            ],
            "description": "Hydroxylation of diol-diterpene peregrinol",
            "reactionSMARTS": "[#6:1]-[#6:2](-[#6:6]-[#6:7]-[#6@@:8]1(-[#6@:21]2(/[#6:22])-[#6@:14](-[#6:15](-[#6:18]-[#6:19]-[#6:20]-2)(-[#6:17])-[#6:16])/[#6:13]-[#6:12]-[#6@:10]-1\\[#6:11])\\[#8:9])=[#6:3]-[#6:4]-[#8:5]>>[#6:11]/[#6@:10]1-[#6@@:8](-[#6:7]-[#6:6]-[#6:2](-[#6:1]-[#8])=[#6:3]-[#6:4]-[#8:5])(/[#8:9])-[#6@:21]2(\\[#6:22])-[#6@:14](-[#6:15](-[#6:18]-[#6:19]-[#6:20]-2)(-[#6:16])-[#6:17])\\[#6:13]-[#6:12]-1",
            "reactions": [
                {
                    "substrate": "C/C(=C\\CO)CC[C@@]1(O)[C@H](C)CC[C@H]2C(C)(C)CCC[C@@]21C",
                    "products": [
                        "C[C@@H]1CC[C@H]2C(C)(C)CCC[C@]2(C)[C@@]1(O)CCC(=CCO)CO"
                    ],
                    "isIntermediate": true,
                    "description": "16-hydroxylation of peregrinol to labd-13Z-ene-9,15,16-triol"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1111/tpj.13822"
                ]
            },
            "databaseIds": {
                "rhea": "62192"
            }
        }
    ]
}