{
    "accession": "MITE0000409",
    "status": "active",
    "comment": "P450 hydroxylase",
    "changelog": [
        {
            "version": "1",
            "date": "2026-06-09",
            "contributors": [
                "0009-0008-8086-5325"
            ],
            "reviewers": [
                "0000-0001-5701-0590"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "Luz25",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1002/anie.202105312"
        ],
        "databaseIds": {
            "uniprot": "UPI001F56E2E2",
            "genpept": "MCK2216164.1"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": " Luz25 is a P450 hydroxylase responsible for beta-hydroxylation at the N-Methylvaline residues of the luzopeptin scaffold",
            "reactionSMARTS": "[#6:1]1-[#6:83]-[#6:82]-[#7:81]-[#7:80]2-[#6:2]-1-[#6:3](=[#8:92])-[#7:4]-[#6:5]-[#6:6](=[#8:91])-[#7:7](-[#6:9]-[#6:10](=[#8:90])-[#7:11](-[#6:13](-[#6:17](=[#8:89])-[#8:18]-[#6:19]-[#6:20](-[#6:37](=[#8:88])-[#7:38]1-[#6:43](-[#6:44](=[#8:87])-[#7:45]-[#6:46]-[#6:47](=[#8:86])-[#7:48](-[#6:50]-[#6:51](=[#8:85])-[#7:52](-[#6:54](-[#6:58](=[#8:84])-[#8:59]-[#6:60]-[#6:61](-[#6:78]-2=[#8:79])-[#7:62]-[#6:63](=[#8:77])-[#6:64]2:[#6:75](-[#8:76]):[#6:74]:[#6:67]3:[#6:68]:[#6:69](:[#6:72]:[#6:73]:[#6:66]:3:[#7:65]:2)-[#8:70]-[#6:71])-[#6:55](-[#6:57])-[#6:56])-[#6:53])-[#6:49])-[#6:42]-[#6:41]-[#6:40]-[#7:39]-1)-[#7:21]-[#6:22](=[#8:36])-[#6:23]1:[#6:34](-[#8:35]):[#6:33]:[#6:26]2:[#6:27]:[#6:28](:[#6:31]:[#6:32]:[#6:25]:2:[#7:24]:1)-[#8:29]-[#6:30])-[#6:14](-[#6:16])-[#6:15])-[#6:12])-[#6:8]>>[#6:1]1-[#6:83]-[#6:82]-[#7:81]-[#7:80]2-[#6:2]-1-[#6:3](=[#8:92])-[#7:4]-[#6:5]-[#6:6](=[#8:91])-[#7:7](-[#6:9]-[#6:10](=[#8:90])-[#7:11](-[#6:13](-[#6:17](=[#8:89])-[#8:18]-[#6:19]-[#6:20](-[#6:37](=[#8:88])-[#7:38]1-[#6:43](-[#6:44](=[#8:87])-[#7:45]-[#6:46]-[#6:47](=[#8:86])-[#7:48](-[#6:50]-[#6:51](=[#8:85])-[#7:52](-[#6:54](-[#6:58](=[#8:84])-[#8:59]-[#6:60]-[#6:61](-[#6:78]-2=[#8:79])-[#7:62]-[#6:63](=[#8:77])-[#6:64]2:[#6:75](-[#8:76]):[#6:74]:[#6:67]3:[#6:68]:[#6:69](:[#6:72]:[#6:73]:[#6:66]:3:[#7:65]:2)-[#8:70]-[#6:71])-[#6:55](-[#8])(-[#6:57])-[#6:56])-[#6:53])-[#6:49])-[#6:42]-[#6:41]-[#6:40]-[#7:39]-1)-[#7:21]-[#6:22](=[#8:36])-[#6:23]1:[#6:34](-[#8:35]):[#6:33]:[#6:26]2:[#6:27]:[#6:28](:[#6:31]:[#6:32]:[#6:25]:2:[#7:24]:1)-[#8:29]-[#6:30])-[#6:14](-[#8])(-[#6:16])-[#6:15])-[#6:12])-[#6:8]",
            "reactions": [
                {
                    "substrate": "COc1ccc2nc(C(=O)NC3COC(=O)C(C(C)C)N(C)C(=O)CN(C)C(=O)CNC(=O)C4CCCNN4C(=O)C(NC(=O)c4nc5ccc(OC)cc5cc4O)COC(=O)C(C(C)C)N(C)C(=O)CN(C)C(=O)CNC(=O)C4CCCNN4C3=O)c(O)cc2c1",
                    "products": [
                        "COc1ccc2nc(C(=O)NC3COC(=O)C(C(C)(C)O)N(C)C(=O)CN(C)C(=O)CNC(=O)C4CCCNN4C(=O)C(NC(=O)c4nc5ccc(OC)cc5cc4O)COC(=O)C(C(C)(C)O)N(C)C(=O)CN(C)C(=O)CNC(=O)C4CCCNN4C3=O)c(O)cc2c1"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1002/anie.202105312"
                ]
            }
        }
    ]
}