{
    "accession": "MITE0000408",
    "status": "active",
    "comment": "P450-mediated hydroxylation and carbon\u2013nitrogen double bond formation in the luzopeptins biosynthesis",
    "changelog": [
        {
            "version": "1",
            "date": "2026-06-09",
            "contributors": [
                "0009-0008-8086-5325"
            ],
            "reviewers": [
                "0000-0001-5701-0590"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "Luz26",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1002/anie.202105312"
        ],
        "databaseIds": {
            "uniprot": "UPI001F59E914",
            "genpept": "MCK2216163.1"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Dehydrogenation",
                "Hydroxylation",
                "Other"
            ],
            "description": "Luz26 is a multitasking tailoring P450 that catalyzes both hydroxylations and C-N bond desaturations to furnish the 4-OH-Thp subunits resulting in di-deacetylated derivative luzopeptin C",
            "reactionSMARTS": "[#6:1]1-[#6:6]2-[#6:7](-[#7:9]-[#6:10]-[#6:11](-[#7:13](-[#6:94])-[#6:14]-[#6:15](-[#7:17](-[#6:93])-[#6:18](-[#6:89](-[#6:92])(-[#6:91])-[#8:90])-[#6:19](-[#8:21]-[#6:22]-[#6:23](-[#7:73]-[#6:74](-[#6:76]3:[#7:86]:[#6:85]4:[#6:80](:[#6:81]:[#6:82](-[#8:87]-[#6:88]):[#6:83]:[#6:84]:4):[#6:79]:[#6:77]:3-[#8:78])=[#8:75])-[#6:24](-[#7:26]3-[#7:72]-[#6:71]-[#6:70]-[#6:69]-[#6:27]-3-[#6:28](-[#7:30]-[#6:31]-[#6:32](-[#7:34](-[#6:68])-[#6:35]-[#6:36](-[#7:38](-[#6:67])-[#6:39](-[#6:63](-[#6:66])(-[#6:65])-[#8:64])-[#6:40](-[#8:42]-[#6:43]-[#6:44](-[#7:47]-[#6:48](-[#6:50]3:[#7:60]:[#6:59]4:[#6:54](:[#6:55]:[#6:56](-[#8:61]-[#6:62]):[#6:57]:[#6:58]:4):[#6:53]:[#6:51]:3-[#8:52])=[#8:49])-[#6:45](=[#8:46])-[#7:5]-2-[#7:4]-[#6:3]-[#6:2]-1)=[#8:41])=[#8:37])=[#8:33])=[#8:29])=[#8:25])=[#8:20])=[#8:16])=[#8:12])=[#8:8]>>[#6:1]1(-[#8])-[#6:6]2-[#6:7](-[#7:9]-[#6:10]-[#6:11](-[#7:13](-[#6:94])-[#6:14]-[#6:15](-[#7:17](-[#6:93])-[#6:18](-[#6:89](-[#6:92])(-[#6:91])-[#8:90])-[#6:19](-[#8:21]-[#6:22]-[#6:23](-[#7:73]-[#6:74](-[#6:76]3:[#7:86]:[#6:85]4:[#6:80](:[#6:81]:[#6:82](-[#8:87]-[#6:88]):[#6:83]:[#6:84]:4):[#6:79]:[#6:77]:3-[#8:78])=[#8:75])-[#6:24](-[#7:26]3-[#7:72]=[#6:71]-[#6:70]-[#6:69](-[#8])-[#6:27]-3-[#6:28](-[#7:30]-[#6:31]-[#6:32](-[#7:34](-[#6:68])-[#6:35]-[#6:36](-[#7:38](-[#6:67])-[#6:39](-[#6:63](-[#6:66])(-[#6:65])-[#8:64])-[#6:40](-[#8:42]-[#6:43]-[#6:44](-[#7:47]-[#6:48](-[#6:50]3:[#7:60]:[#6:59]4:[#6:54](:[#6:55]:[#6:56](-[#8:61]-[#6:62]):[#6:57]:[#6:58]:4):[#6:53]:[#6:51]:3-[#8:52])=[#8:49])-[#6:45](=[#8:46])-[#7:5]-2-[#7:4]=[#6:3]-[#6:2]-1)=[#8:41])=[#8:37])=[#8:33])=[#8:29])=[#8:25])=[#8:20])=[#8:16])=[#8:12])=[#8:8]",
            "reactions": [
                {
                    "substrate": "COc1ccc2nc(C(=O)NC3COC(=O)C(C(C)(C)O)N(C)C(=O)CN(C)C(=O)CNC(=O)C4CCCNN4C(=O)C(NC(=O)c4nc5ccc(OC)cc5cc4O)COC(=O)C(C(C)(C)O)N(C)C(=O)CN(C)C(=O)CNC(=O)C4CCCNN4C3=O)c(O)cc2c1",
                    "products": [
                        "COc1ccc2nc(C(=O)NC3COC(=O)C(C(C)(C)O)N(C)C(=O)CN(C)C(=O)CNC(=O)C4C(O)CC=NN4C(=O)C(NC(=O)c4nc5ccc(OC)cc5cc4O)COC(=O)C(C(C)(C)O)N(C)C(=O)CN(C)C(=O)CNC(=O)C4C(O)CC=NN4C3=O)c(O)cc2c1"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1002/anie.202105312"
                ]
            }
        }
    ]
}