{
    "accession": "MITE0000407",
    "status": "active",
    "comment": "Luz27 is a membrane-bound acyltransferase that mediates the subsequent O-acetylation extracellularly.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-06-09",
            "contributors": [
                "0009-0008-8086-5325"
            ],
            "reviewers": [
                "0000-0001-5701-0590"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "Luz27",
        "description": "Acetyltransferase",
        "references": [
            "doi:10.1002/anie.202105312"
        ],
        "databaseIds": {
            "uniprot": "UPI001F57AB4F",
            "genpept": "MCK2216162.1"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Acetylation"
            ],
            "description": "Luz27 performs stepwise O-acetylations on the 4-OH groups of 4-OH-Thp residues to afford a final product, luzopeptin A",
            "reactionSMARTS": "[#6:1]1(-[#6:87]-[#6:86]=[#7:85]-[#7:84]2-[#6:3]-1-[#6:4](=[#8:96])-[#7:5]-[#6:6]-[#6:7](=[#8:95])-[#7:8](-[#6:10]-[#6:11](=[#8:94])-[#7:12](-[#6:14](-[#6:19](=[#8:93])-[#8:20]-[#6:21]-[#6:22](-[#6:39](=[#8:92])-[#7:40]1-[#6:46](-[#6:47](=[#8:91])-[#7:48]-[#6:49]-[#6:50](=[#8:90])-[#7:51](-[#6:53]-[#6:54](=[#8:89])-[#7:55](-[#6:57](-[#6:62](=[#8:88])-[#8:63]-[#6:64]-[#6:65](-[#6:82]-2=[#8:83])-[#7:66]-[#6:67](=[#8:81])-[#6:68]2:[#6:79](-[#8:80]):[#6:78]:[#6:71]3:[#6:72]:[#6:73](:[#6:76]:[#6:77]:[#6:70]:3:[#7:69]:2)-[#8:74]-[#6:75])-[#6:58](-[#8:61])(-[#6:60])-[#6:59])-[#6:56])-[#6:52])-[#6:44](-[#8:45])-[#6:43]-[#6:42]=[#7:41]-1)-[#7:23]-[#6:24](=[#8:38])-[#6:25]1:[#6:36](-[#8:37]):[#6:35]:[#6:28]2:[#6:29]:[#6:30](:[#6:33]:[#6:34]:[#6:27]:2:[#7:26]:1)-[#8:31]-[#6:32])-[#6:15](-[#8:18])(-[#6:17])-[#6:16])-[#6:13])-[#6:9])-[#8:2]>>[#6:1]1(-[#6:87]-[#6:86]=[#7:85]-[#7:84]2-[#6:3]-1-[#6:4](=[#8:96])-[#7:5]-[#6:6]-[#6:7](=[#8:95])-[#7:8](-[#6:10]-[#6:11](=[#8:94])-[#7:12](-[#6:14](-[#6:19](=[#8:93])-[#8:20]-[#6:21]-[#6:22](-[#6:39](=[#8:92])-[#7:40]1-[#6:46](-[#6:47](=[#8:91])-[#7:48]-[#6:49]-[#6:50](=[#8:90])-[#7:51](-[#6:53]-[#6:54](=[#8:89])-[#7:55](-[#6:57](-[#6:62](=[#8:88])-[#8:63]-[#6:64]-[#6:65](-[#6:82]-2=[#8:83])-[#7:66]-[#6:67](=[#8:81])-[#6:68]2:[#6:79](-[#8:80]):[#6:78]:[#6:71]3:[#6:72]:[#6:73](:[#6:76]:[#6:77]:[#6:70]:3:[#7:69]:2)-[#8:74]-[#6:75])-[#6:58](-[#8:61])(-[#6:60])-[#6:59])-[#6:56])-[#6:52])-[#6:44](-[#8:45]-[#6:24](=[#8:38])-[#6:25])-[#6:43]-[#6:42]=[#7:41]-1)-[#7:23]-[#6:24](=[#8:38])-[#6:25]1:[#6:36](-[#8:37]):[#6:35]:[#6:28]2:[#6:29]:[#6:30](:[#6:33]:[#6:34]:[#6:27]:2:[#7:26]:1)-[#8:31]-[#6:32])-[#6:15](-[#8:18])(-[#6:17])-[#6:16])-[#6:13])-[#6:9])-[#8:2]-[#6:67](=[#8:81])-[#6:68]",
            "reactions": [
                {
                    "substrate": "COc1ccc2nc(C(=O)NC3COC(=O)C(C(C)(C)O)N(C)C(=O)CN(C)C(=O)CNC(=O)C4C(O)CC=NN4C(=O)C(NC(=O)c4nc5ccc(OC)cc5cc4O)COC(=O)C(C(C)(C)O)N(C)C(=O)CN(C)C(=O)CNC(=O)C4C(O)CC=NN4C3=O)c(O)cc2c1",
                    "products": [
                        "COc1ccc2nc(C(=O)NC3COC(=O)C(C(C)(C)O)N(C)C(=O)CN(C)C(=O)CNC(=O)C4C(OC(C)=O)CC=NN4C(=O)C(NC(=O)c4nc5ccc(OC)cc5cc4O)COC(=O)C(C(C)(C)O)N(C)C(=O)CN(C)C(=O)CNC(=O)C4C(OC(C)=O)CC=NN4C3=O)c(O)cc2c1"
                    ],
                    "isIntermediate": false,
                    "description": "Luz27, a membrane-bound acyltransferase performs stepwise O-acetylations at the 4-OH groups in di-deacetylated derivative luzopeptin C to afford luzopeptin A, using acetyl-CoA as the acetyl donor"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1002/anie.202105312"
                ]
            }
        }
    ]
}