{
    "accession": "MITE0000400",
    "status": "active",
    "comment": "Monensin hydroxylase",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-27",
            "contributors": [
                "0009-0008-8086-5325"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "MonD",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1046/j.1365-2958.2003.03571.x",
            "doi:10.3762/bjoc.10.34"
        ],
        "databaseIds": {
            "uniprot": "UPI000005D0CA",
            "genpept": "AAO65808.1",
            "mibig": "BGC0000100"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Hydroxylation"
            ],
            "description": "The preferred order of events in monensin biosynthesis is hydroxylation at C-26 catalysed by MonD, followed by O-methylation at the hydroxy group at C-3 catalysed by MonE",
            "reactionSMARTS": "[#6:1]-[#6:2]-[#6:3]1(-[#6:30]2-[#6:34](-[#6:35])-[#6:33]-[#6:32](-[#6:36]3-[#6:44](-[#6:45])-[#6:43]-[#6:41](-[#6:42])-[#6:38](-[#6:40])(-[#8:39])-[#8:37]-3)-[#8:31]-2)-[#8:7]-[#6:6](-[#6:8]2(-[#8:29]-[#6:12]3(-[#8:19]-[#6:18](-[#6:20](-[#6:22](-[#6:24](-[#6:26](=[#8:28])-[*])-[#6:25])-[#8:23])-[#6:21])-[#6:16](-[#6:17])-[#6:14](-[#8:15])-[#6:13]-3)-[#6:11]-[#6:10]-2)-[#6:9])-[#6:5]-[#6:4]-1>>[#6:1]-[#6:2]-[#6:3]1(-[#6:30]2-[#6:34](-[#6:35])-[#6:33]-[#6:32](-[#6:36]3-[#6:44](-[#6:45])-[#6:43]-[#6:41](-[#6:42])-[#6:38](-[#6:40]-[#8])(-[#8:39])-[#8:37]-3)-[#8:31]-2)-[#8:7]-[#6:6](-[#6:8]2(-[#8:29]-[#6:12]3(-[#8:19]-[#6:18](-[#6:20](-[#6:22](-[#6:24](-[#6:26](=[#8:28])-[*])-[#6:25])-[#8:23])-[#6:21])-[#6:16](-[#6:17])-[#6:14](-[#8:15])-[#6:13]-3)-[#6:11]-[#6:10]-2)-[#6:9])-[#6:5]-[#6:4]-1",
            "reactions": [
                {
                    "substrate": "*C(=O)C(C)C(O)C(C)C1OC2(CCC(C)(C3CCC(CC)(C4OC(C5OC(C)(O)C(C)CC5C)CC4C)O3)O2)CC(O)C1C",
                    "products": [
                        "*C(=O)C(C)C(O)C(C)C1OC2(CCC(C)(C3CCC(CC)(C4OC(C5OC(O)(CO)C(C)CC5C)CC4C)O3)O2)CC(O)C1C"
                    ],
                    "isIntermediate": true,
                    "description": "MonD catalyzes hydroxylation at C-26 of dehydroxydemethylmonensin to produce intermediate demethylmonensin"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Inference from genomic data and chemical structure"
                ],
                "references": [
                    "doi:10.1046/j.1365-2958.2003.03571.x",
                    "doi:10.3762/bjoc.10.34"
                ]
            }
        }
    ]
}