{
    "accession": "MITE0000399",
    "status": "active",
    "comment": "Monensin 3-O-methyl transferase (SAM-dependent methyltransferase superfamily)",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-27",
            "contributors": [
                "0009-0008-8086-5325"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 1 added cofactor information."
        }
    ],
    "enzyme": {
        "name": "MonE",
        "description": "O-methyltransferase",
        "references": [
            "doi:10.1046/j.1365-2958.2003.03571.x",
            "doi:10.3762/bjoc.10.34"
        ],
        "databaseIds": {
            "uniprot": "UPI000005D0C6",
            "genpept": "AAO65792.1",
            "mibig": "BGC0000100"
        },
        "cofactors": {
            "organic": [
                "SAM"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "MonE catalyzes O-methylation of the hydroxy group at C-3 of demethylmonensin",
            "reactionSMARTS": "[#6:1]-[#6:2]-[#6:3]1(-[#6:30]2-[#6:34](-[#6:35])-[#6:33]-[#6:32](-[#6:36]3-[#6:45](-[#6:46])-[#6:44]-[#6:42](-[#6:43])-[#6:38](-[#6:40]-[#8:41])(-[#8:39])-[#8:37]-3)-[#8:31]-2)-[#8:7]-[#6:6](-[#6:8]2(-[#8:29]-[#6:12]3(-[#8:19]-[#6:18](-[#6:20](-[#6:22](-[#6:24](-[#6:26](=[#8:28])-[*])-[#6:25])-[#8:23])-[#6:21])-[#6:16](-[#6:17])-[#6:14](-[#8:15])-[#6:13]-3)-[#6:11]-[#6:10]-2)-[#6:9])-[#6:5]-[#6:4]-1>>[#6:1]-[#6:2]-[#6:3]1(-[#6:30]2-[#6:34](-[#6:35])-[#6:33]-[#6:32](-[#6:36]3-[#6:45](-[#6:46])-[#6:44]-[#6:42](-[#6:43])-[#6:38](-[#6:40]-[#8:41])(-[#8:39])-[#8:37]-3)-[#8:31]-2)-[#8:7]-[#6:6](-[#6:8]2(-[#8:29]-[#6:12]3(-[#8:19]-[#6:18](-[#6:20](-[#6:22](-[#6:24](-[#6:26](=[#8:28])-[*])-[#6:25])-[#8:23]-[#6])-[#6:21])-[#6:16](-[#6:17])-[#6:14](-[#8:15])-[#6:13]-3)-[#6:11]-[#6:10]-2)-[#6:9])-[#6:5]-[#6:4]-1",
            "reactions": [
                {
                    "substrate": "*C(=O)C(C)C(O)C(C)C1OC2(CCC(C)(C3CCC(CC)(C4OC(C5OC(O)(CO)C(C)CC5C)CC4C)O3)O2)CC(O)C1C",
                    "products": [
                        "*C(=O)C(C)C(OC)C(C)C1OC2(CCC(C)(C3CCC(CC)(C4OC(C5OC(O)(CO)C(C)CC5C)CC4C)O3)O2)CC(O)C1C"
                    ],
                    "isIntermediate": true,
                    "description": "After hydroxylation by MonD and O-methylation by MonE, the ACP-bound product is hydrolysed by MonCII (thioesterase) to release free monensin A"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Inference from genomic data and chemical structure",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1046/j.1365-2958.2003.03571.x",
                    "doi:10.3762/bjoc.10.34",
                    "doi:10.1038/s41467-023-41889-0"
                ]
            }
        }
    ]
}