{
    "accession": "MITE0000397",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-06",
            "contributors": [
                "0000-0002-4540-6501"
            ],
            "reviewers": [
                "0000-0001-5863-7248"
            ],
            "comment": "New entry. Reviewer 1 changed reaction term, added FAD cofactor, corrected intermediate to true."
        }
    ],
    "enzyme": {
        "name": "PtmO4",
        "description": "long-chain acyl-CoA dehydrogenase",
        "references": [
            "doi:10.1039/C5MB00303B"
        ],
        "databaseIds": {
            "uniprot": "D8L2U8",
            "genpept": "ACO31278.1"
        },
        "cofactors": {
            "organic": [
                "FAD"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Dehydrogenation"
            ],
            "description": "long-chain acyl-CoA dehydrogenase, is responsible for the formation of the double bond between C3 and C4",
            "reactionSMARTS": "[#6:1]/[#6@:2](-[#6:22]=[#8:23])-[#6:3]-[#6:4]-[#6:5]-[#6@@:6]1(-[#6@:21]2-[#6@:12]3(-[#6:20]-[#6@:18]4(/[#6:19])-[#6@@:14](-[#6:15]\\[#6@@:16]\\2-[#8:17]-4)\\[#6:13]-3)\\[#6:11]=[#6:10]-[#6:8]-1=[#8:9])/[#6:7]>>[#6:1]-[#6:2](-[#6:22]=[#8:23])=[#6:3]-[#6:4]-[#6:5]-[#6@@:6]1(-[#6@:21]2-[#6@:12]3(-[#6:20]-[#6@:18]4(/[#6:19])-[#6@@:14](-[#6:15]\\[#6@@:16]\\2-[#8:17]-4)\\[#6:13]-3)\\[#6:11]=[#6:10]-[#6:8]-1=[#8:9])/[#6:7]",
            "reactions": [
                {
                    "substrate": "*C(=O)[C](C)CCC[C@]1(C)[C]2[C]3C[C]4C[C@]2(C=CC1=O)C[C@@]4(C)O3",
                    "products": [
                        "*C(=O)C(C)=CCC[C@@]1(C)C(=O)C=C[C@@]23CC4CC(O[C@]4(C)C2)C31"
                    ],
                    "isIntermediate": true
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1039/C5MB00303B"
                ]
            }
        }
    ]
}