{
    "accession": "MITE0000384",
    "status": "active",
    "comment": "SAM-dependent N-methyltransferase",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-20",
            "contributors": [
                "0009-0008-8086-5325"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "Ind7",
        "description": "N-methyltransferase",
        "references": [
            "doi:10.1073/pnas.1419964112"
        ],
        "databaseIds": {
            "uniprot": "A0A0D4BSP3",
            "genpept": "AJT38688.1",
            "mibig": "BGC0001206"
        },
        "cofactors": {
            "organic": [
                "SAM"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "Ind7 (N-methyltransferase) catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (SAM) to N-demethylindolmycin, producing mature indolmycin.",
            "reactionSMARTS": "[#6:1]\\[#6@@:2](-[#6:10]1:[#6:13]2:[#6:18]:[#6:17]:[#6:16]:[#6:15]:[#6:14]:2:[#7:12]:[#6:11]:1)-[#6@@:3]1-[#6:8](=[#8:9])-[#7:7]=[#6:5](-[#7:6])-[#8:4]/1>>[#6]-[#7:6]-[#6:5]1-[#8:4]-[#6@:3](-[#6@@:2](\\[#6:1])-[#6:10]2:[#6:13]3:[#6:18]:[#6:17]:[#6:16]:[#6:15]:[#6:14]:3:[#7:12]:[#6:11]:2)/[#6:8](=[#8:9])-[#7:7]=1",
            "reactions": [
                {
                    "substrate": "C[C@@H](c1c[nH]c2ccccc12)[C@H]1OC(N)=NC1=O",
                    "products": [
                        "CNC1=NC(=O)[C@@H]([C@@H](C)c2c[nH]c3ccccc23)O1"
                    ],
                    "isIntermediate": false,
                    "description": "N-methylation of N-demethylindolmycin to indolmycin"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1073/pnas.1419964112"
                ]
            }
        }
    ]
}