{
    "accession": "MITE0000383",
    "status": "active",
    "comment": "GroI oxidizes the allylic C-17 position of gromomycin C to form a keto group",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-24",
            "contributors": [
                "0009-0008-8086-5325"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "GroI",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1002/anie.202422270"
        ],
        "databaseIds": {
            "uniprot": "UPI0034576DF7"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Monooxygenation"
            ],
            "description": "GroI incorporates a keto group at C-17 of gromomycin C, leading to the formation of gromomycin B",
            "reactionSMARTS": "[#7:1]=[#6:2]1-[#7:37]-[#6:36]=[#6:6]2-[#6:7]3(-[#6:31](-[#6:33]-[#6:34](-[#8:35])-[#6:4]-2(-[#6:5])-[#7:3]-1)-[#6:12]1(-[#6:14]-[#6:15]-[#6:16]2-[#6:22](-[#6:29])(-[#6:23]-[#6:24]-[#6:25]=[#6:26](-[#6:28])-[#6:27])-[#6:21]-[#6:20]-[#6:19]=[#6:18]-2-[#6:10]-1(-[#6:11])-[#6:9]-[#6:8]-3)-[#6:13])-[#6:30]>>[#7:1]=[#6:2]1-[#7:37]-[#6:36]=[#6:6]2-[#6:7]3(-[#6:31](-[#6:33]-[#6:34](-[#8:35])-[#6:4]-2(-[#6:5])-[#7:3]-1)-[#6:12]1(-[#6:14]-[#6:15]-[#6:16]2-[#6:22](-[#6:29])(-[#6:23]-[#6:24]-[#6:25]=[#6:26](-[#6:27])-[#6:28])-[#6:21]-[#6:20](=[#8])-[#6:19]=[#6:18]-2-[#6:10]-1(-[#6:11])-[#6:9]-[#6:8]-3)-[#6:13])-[#6:30]",
            "reactions": [
                {
                    "substrate": "CC(C)=CCCC1(C)CCC=C2C1CCC1(C)C3CC(O)C4(C)NC(=N)NC=C4C3(C)CCC21C",
                    "products": [
                        "CC(C)=CCCC1(C)CC(=O)C=C2C1CCC1(C)C3CC(O)C4(C)NC(=N)NC=C4C3(C)CCC21C"
                    ],
                    "isIntermediate": true,
                    "description": "GroI incorporates a keto group at C-17 of gromomycin C, leading to the formation of gromomycin B"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1002/anie.202422270"
                ]
            }
        }
    ]
}