{
    "accession": "MITE0000382",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-16",
            "contributors": [
                "0000-0002-0771-9611"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 1 added RHEA ID and EC number, added cofactors, improved reaction description."
        }
    ],
    "enzyme": {
        "name": "AscJ",
        "description": "Short-chain dehydrogenase/reductase",
        "references": [
            "doi:10.1073/pnas.1819254116"
        ],
        "databaseIds": {
            "uniprot": "A0A455R5K2",
            "genpept": "BBF25320.1",
            "mibig": "BGC0001924"
        },
        "cofactors": {
            "organic": [
                "NADP"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Oxidation"
            ],
            "description": "Oxidizes intermediate meroterpenoid in the ascofuranone biosynthetic pathway, leading to mature ascofuranone.",
            "reactionSMARTS": "[#6]-[#6](-[#6]-[#6]-[#6]=[#6](-[#6]1-[#8]-[#6](-[#6])(-[#6])-[#6@@](/[#8])-[#6]-1)-[#6])=[#6]-[#6]-[#6]1:[#6](-[#8]):[#6](-[#6]=[#8]):[#6](-[#6]):[#6](-[Cl]):[#6]:1-[#8;-]>>[#6]1(-[#8;-]):[#6](-[#6]-[#6]=[#6](-[#6])-[#6]-[#6]-[#6]=[#6](-[#6]2-[#8]-[#6](-[#6])(-[#6])-[#6](=[#8])-[#6]-2)-[#6]):[#6](-[#8]):[#6](-[#6]=[#8]):[#6](-[#6]):[#6]:1-[Cl]",
            "reactions": [
                {
                    "substrate": "CC(=CCc1c([O-])c(Cl)c(C)c(C=O)c1O)CCC=C(C)C1C[C@H](O)C(C)(C)O1",
                    "products": [
                        "CC(=CCc1c([O-])c(Cl)c(C)c(C=O)c1O)CCC=C(C)C1CC(=O)C(C)(C)O1"
                    ],
                    "isIntermediate": false,
                    "description": "Converts Ascofuranol to Ascofuranone by oxidation."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1073/pnas.1819254116"
                ]
            },
            "databaseIds": {
                "rhea": "63112",
                "ec": "1.1.99"
            }
        }
    ]
}