{
    "accession": "MITE0000381",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-16",
            "contributors": [
                "0000-0002-0771-9611"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 1 fixed reaction SMARTS and product, added RHEA ID and EC number, improved reaction descriptions."
        }
    ],
    "enzyme": {
        "name": "AscI",
        "description": "Terpene cyclase",
        "references": [
            "doi:10.1073/pnas.1819254116"
        ],
        "databaseIds": {
            "uniprot": "A0A455R4Z0",
            "genpept": "BBF25321.1",
            "mibig": "BGC0001924"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Cyclization"
            ],
            "description": "Cyclization of meroterpenoid intermediate in the ascofuranone biosynthetic pathway.",
            "reactionSMARTS": "[#6]1(-[#8]):[#6](-[#6]-[#6]=[#6](-[#6])-[#6]-[#6]-[#6]=[#6](-[#6])-[#6@@](/[#8])-[#6]\\[#6@@]2-[#8]-[#6]-2(-[#6])-[#6]):[#6](-[#8]):[#6](-[#6]=[#8]):[#6](-[#6]):[#6]:1-[Cl]>>[#6]1(-[#8]):[#6](-[#6]-[#6]=[#6](-[#6])-[#6]-[#6]-[#6]=[#6](-[#6]2-[#8]-[#6](-[#6])(-[#6])-[#6@@](/[#8])-[#6]-2)-[#6]):[#6](-[#8]):[#6](-[#6]=[#8]):[#6](-[#6]):[#6]:1-[Cl]",
            "reactions": [
                {
                    "substrate": "C/C(=C\\Cc1c(O)c(Cl)c(C)c(C=O)c1O)CC/C=C(\\C)[C@@H](O)C[C@@H]1OC1(C)C",
                    "products": [
                        "CC(=CCc1c(O)c(Cl)c(C)c(C=O)c1O)CCC=C(C)C1C[C@H](O)C(C)(C)O1"
                    ],
                    "isIntermediate": true,
                    "description": "Converts 16-hydroxy-ilicicolin A epoxide to ascofuranol by cyclisation to form a tetrahydrofuran ring."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1073/pnas.1819254116"
                ]
            },
            "databaseIds": {
                "rhea": "63108",
                "ec": "5.4.99"
            }
        }
    ]
}