{
    "accession": "MITE0000377",
    "status": "active",
    "comment": "Bifunctional cytochrome P450/NADPH P450 reductase",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-16",
            "contributors": [
                "0000-0002-0771-9611"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 1 improved reaction description, added RHEA crossref, specified Fe, Heme as cofactors."
        }
    ],
    "enzyme": {
        "name": "AscE",
        "description": "Cytochrome P450/NADPH P450 reductase",
        "references": [
            "doi:10.1073/pnas.1819254116"
        ],
        "databaseIds": {
            "uniprot": "A0A455R5H4",
            "genpept": "BBF25317.1",
            "mibig": "BGC0001923"
        },
        "cofactors": {
            "organic": [
                "Heme",
                "NADP"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Oxidation"
            ],
            "description": "Epoxidation in intermediate meroterpenoid in the ascochlorin/ascofuranone biosynthetic pathway.",
            "reactionSMARTS": "[#6]-[#6](=[#6]-[#6]-[#6]-[#6](=[#6]-[#6]-[#6]-[#6](=[#6]-[#6]-[#6]1:[#6](-[#8]):[#6](-[#6]=[#8]):[#6](-[#6]):[#6](-[Cl]):[#6]:1-[#8])-[#6])-[#6])-[#6]>>[#6]-[#6]1(-[#8]\\[#6@@]-1-[#6]-[#6]-[#6](=[#6]-[#6]-[#6]-[#6](=[#6]-[#6]-[#6]1:[#6](-[#8]):[#6](-[#6]=[#8]):[#6](-[#6]):[#6](-[Cl]):[#6]:1-[#8])-[#6])-[#6])-[#6]",
            "reactions": [
                {
                    "substrate": "CC(C)=CCCC(C)=CCCC(C)=CCc1c(O)c(Cl)c(C)c(C=O)c1O",
                    "products": [
                        "CC(=CCc1c(O)c(Cl)c(C)c(C=O)c1O)CCC=C(C)CC[C@H]1OC1(C)C"
                    ],
                    "isIntermediate": true,
                    "description": "Converts Ilicicolin A to Ilicicolin A epoxide by stereoselective epoxidation of the terminal double bond of the prenyl group."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1073/pnas.1819254116"
                ]
            },
            "databaseIds": {
                "rhea": "63092",
                "ec": "1.14.14"
            }
        }
    ]
}