{
    "accession": "MITE0000376",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-16",
            "contributors": [
                "0000-0002-0771-9611"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 1 improved reaction descriptions, added RHEA cross-reference"
        }
    ],
    "enzyme": {
        "name": "AscD",
        "description": "Flavin-dependent halogenase",
        "references": [
            "doi:10.1073/pnas.1819254116"
        ],
        "databaseIds": {
            "uniprot": "A0A455R7M0",
            "genpept": "BBF25316.1",
            "mibig": "BGC0001923"
        },
        "cofactors": {
            "organic": [
                "FAD"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Halogenation"
            ],
            "description": "Halogenation of meroterpenoid precursor in the ascochlorin/ascofuranone biosynthetic pathway.",
            "reactionSMARTS": "[#6]-[#6](=[#6]-[#6]-[#6]-[#6](=[#6]-[#6]-[#6]-[#6](=[#6]-[#6]-[#6]1:[#6](-[#8]):[#6](-[#6]=[#8]):[#6](-[#6]):[#6]:[#6]:1-[#8])-[#6])-[#6])-[#6]>>[#6]-[#6](=[#6]-[#6]-[#6]-[#6](=[#6]-[#6]-[#6]-[#6](=[#6]-[#6]-[#6]1:[#6](-[#8]):[#6](-[#6]=[#8]):[#6](-[#6]):[#6](-[Cl]):[#6]:1-[#8])-[#6])-[#6])-[#6]",
            "reactions": [
                {
                    "substrate": "CC(C)=CCCC(C)=CCCC(C)=CCc1c(O)cc(C)c(C=O)c1O",
                    "products": [
                        "CC(C)=CCCC(C)=CCCC(C)=CCc1c(O)c(Cl)c(C)c(C=O)c1O"
                    ],
                    "isIntermediate": true,
                    "description": "Chlorination of ilicicolin B to give ilicicolin A."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1073/pnas.1819254116"
                ]
            },
            "databaseIds": {
                "rhea": "63084",
                "ec": "1.14.99"
            }
        }
    ]
}