{
    "accession": "MITE0000375",
    "status": "active",
    "comment": "NRPS-like oxidoreductase with carboxylic acid reductase activity.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-16",
            "contributors": [
                "0000-0002-0771-9611"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 1 improved reaction descriptions, added Rhea crossref."
        }
    ],
    "enzyme": {
        "name": "AscB",
        "description": "NRPS-like Oxidoreductase",
        "references": [
            "doi:10.1073/pnas.1819254116"
        ],
        "databaseIds": {
            "uniprot": "A0A455R7E6",
            "genpept": "BBF25314.1",
            "mibig": "BGC0001923"
        },
        "cofactors": {
            "organic": [
                "Adenosine triphosphate",
                "NADP"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Reduction"
            ],
            "description": "Reduces ilicicolinic acid B in the ascochlorin/ascofuranone biosynthetic pathway.",
            "reactionSMARTS": "[#6]-[#6](-[#6])=[#6]-[#6]-[#6]-[#6](-[#6])=[#6]-[#6]-[#6]-[#6](-[#6])=[#6]-[#6]-[#6]1:[#6](-[#8]):[#6]:[#6](-[#6]):[#6](-[#6](=[#8])-[#8]):[#6]:1-[#8]>>[#6]-[#6](-[#6])=[#6]-[#6]-[#6]-[#6](-[#6])=[#6]-[#6]-[#6]-[#6](-[#6])=[#6]-[#6]-[#6]1:[#6](-[#8]):[#6]:[#6](-[#6]):[#6](-[#6]=[#8]):[#6]:1-[#8]",
            "reactions": [
                {
                    "substrate": "CC(C)=CCC/C(C)=C/CC/C(C)=C/Cc1c(O)cc(C)c(C(=O)O)c1O",
                    "products": [
                        "CC(C)=CCCC(C)=CCCC(C)=CCc1c(O)cc(C)c(C=O)c1O"
                    ],
                    "isIntermediate": true,
                    "description": "Reduction of the carboxylic acid to an aldehyde, converting ilicicolinic acid B to ilicicolin B."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1073/pnas.1819254116"
                ]
            },
            "databaseIds": {
                "rhea": "63080"
            }
        }
    ]
}