{
    "accession": "MITE0000367",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-12",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 1 correccted genpept ID, removed uniprot ID, improved descriptions."
        }
    ],
    "enzyme": {
        "name": "LigI",
        "description": "O-methyltransferase",
        "references": [
            "doi:10.1021/jacs.5c03802"
        ],
        "databaseIds": {
            "genpept": "YCL08179.1"
        },
        "cofactors": {
            "organic": [
                "SAM"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "Catalyzes O-methylation of the ring-contracted intermediate in the ligustrone biosynthetic pathway.",
            "reactionSMARTS": "[#8:1]=[#6:2]1-[#6:5]2:[#6:8](:[#6:10]3:[#6:16]4:[#8:17]:[#6:18](:[#6:20]:[#6:21](=[#8:22]):[#6:15]:4:[#6:13](-[#8:14]):[#6:12]:[#6:11]:3:[#6:7]:[#6:6]:2-[#6:4]=[#6:3]-1)-[#6:19])-[#8:9]>>[#8:1]=[#6:2]1-[#6:5]2:[#6:8](:[#6:10]3:[#6:16]4:[#8:17]:[#6:18](:[#6:20]:[#6:21](=[#8:22]):[#6:15]:4:[#6:13](-[#8:14]):[#6:12]:[#6:11]:3:[#6:7]:[#6:6]:2-[#6:4]=[#6:3]-1)-[#6:19])-[#8:9]-[#6]",
            "reactions": [
                {
                    "substrate": "Cc1cc(=O)c2c(O)cc3cc4c(c(O)c3c2o1)C(=O)C=C4",
                    "products": [
                        "COc1c2c(cc3cc(O)c4c(=O)cc(C)oc4c13)C=CC2=O"
                    ],
                    "isIntermediate": false,
                    "description": "Conversion of ring-contracted intermediate to ligustrone A."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1021/jacs.5c03802"
                ]
            }
        }
    ]
}