{
    "accession": "MITE0000366",
    "status": "active",
    "comment": "Catalyzes the oxidative ring contraction",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-12",
            "contributors": [
                "AAAAAAAAAAAAAAAAAAAAAAAA"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 1 corrected genpept accession, removed incorrect uniprot ID, corrected the reaction and substrate, improved descriptions."
        }
    ],
    "enzyme": {
        "name": "LigK",
        "description": "Cytochrome P450",
        "references": [
            "doi:10.1021/jacs.5c03802"
        ],
        "databaseIds": {
            "genpept": "YCL08181.1"
        },
        "cofactors": {
            "organic": [
                "Heme"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Ring contraction"
            ],
            "description": "Oxidative ring contraction of a 1,2-dione in the ligustrone biosynthethic pathway.",
            "reactionSMARTS": "[#6:1]-[#6:2]1:[#8:18]:[#6:17]2:[#6:6](:[#6:7](:[#6:9]:[#6:10]3:[#6:16]:2:[#6:14](-[#8:15]):[#6:13]2-[#6:19](-[#6:21]=[#6:22](-[#8:25])-[#6:23](=[#8:24])-[#6:12]:2:[#6:11]:3)=[#8:20])-[#8:8]):[#6:4](=[#8:5]):[#6:3]:1>>[#6:1]-[#6:2]1:[#8:18]:[#6:17]2:[#6:6](:[#6:7](:[#6:9]:[#6:10]3:[#6:16]:2:[#6:14](-[#8:15]):[#6:13]2-[#6:19](=[#8:20])-[#6:21]=[#6:22]-[#6:12]:2:[#6:11]:3)-[#8:8]):[#6:4](=[#8:5]):[#6:3]:1",
            "reactions": [
                {
                    "substrate": "Cc1cc(=O)c2c(O)cc3cc4c(c(O)c3c2o1)C(=O)C=C(O)C4=O",
                    "products": [
                        "Cc1cc(=O)c2c(O)cc3cc4c(c(O)c3c2o1)C(=O)C=C4"
                    ],
                    "isIntermediate": true,
                    "description": "Oxidative ring contraction of a anthracene dione intermediate in the formation of lugustrone A."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "Inference from genomic data and chemical structure",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1021/jacs.5c03802"
                ]
            }
        }
    ]
}