{
    "accession": "MITE0000363",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-06",
            "contributors": [
                "0009-0003-1714-1678"
            ],
            "reviewers": [
                "0000-0002-7493-459X"
            ],
            "comment": "New entry. Reviewer 1 changed intermediate product to false."
        }
    ],
    "enzyme": {
        "name": "OpaF",
        "description": "O-methyltransferase",
        "references": [
            "doi:10.1038/s41467-020-18713-0"
        ],
        "databaseIds": {
            "uniprot": "A0A0C1E5J2",
            "genpept": "KIA75453.1"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Methylation"
            ],
            "description": "Catalyzes the conversion of Oxepinamide E to the end product Oxepinamide F by methylation of the hydroxyl group at C-12.",
            "reactionSMARTS": "[#6:1]1(=[#8:25])-[#7:2]-[#6:3](-[#6@@:16](\\[#6:19])-[#6:17]-[#6:18])=[#6:4]2-[#7:7]=[#6:8]3-[#8:13]-[#6:15]=[#6:14]-[#6:12]=[#6:11]-[#6@:9]-3(\\[#8:20])-[#6:10](=[#8:21])-[#7:5]-2-[#6@:6]-1\\[#6:22]-[#6:23]1:[#6:28]:[#6:29]:[#6:27]:[#6:26]:[#6:24]:1>>[#6:1]1(=[#8:25])-[#7:2]-[#6:3](-[#6@@:16](\\[#6:19])-[#6:17]-[#6:18])=[#6:4]2-[#7:7]=[#6:8]3-[#8:13]-[#6:15]=[#6:14]-[#6:12]=[#6:11]-[#6@:9]-3(\\[#8:20]-[#6])-[#6:10](=[#8:21])-[#7:5]-2-[#6@:6]-1\\[#6:22]-[#6:23]1:[#6:28]:[#6:29]:[#6:27]:[#6:26]:[#6:24]:1",
            "reactions": [
                {
                    "substrate": "CC[C@H](C)C1=C2N=C3OC=CC=C[C@]3(O)C(=O)N2[C@@H](Cc2ccccc2)C(=O)N1",
                    "products": [
                        "CC[C@H](C)C1=C2N=C3OC=CC=C[C@]3(OC)C(=O)N2[C@@H](Cc2ccccc2)C(=O)N1"
                    ],
                    "isIntermediate": false,
                    "description": "Methylation of the hydroxyl group at C-12."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1038/s41467-020-18713-0"
                ]
            }
        }
    ]
}