{
    "accession": "MITE0000361",
    "status": "active",
    "comment": "Nonheme iron-dependent dioxygenase",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-17",
            "contributors": [
                "0000-0002-0771-9611"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 1 improved reaction descriptions."
        }
    ],
    "enzyme": {
        "name": "AndF",
        "description": "Dioxygenase",
        "references": [
            "doi:10.1021/ja508127q"
        ],
        "databaseIds": {
            "uniprot": "A0A097ZPD9",
            "genpept": "BAP81860.1",
            "mibig": "BGC0002612"
        },
        "cofactors": {
            "organic": [
                "Ascorbic acid",
                "a-Ketoglutaric acid"
            ],
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Dehydrogenation",
                "Dioxygenation",
                "Ring expansion"
            ],
            "description": "Oxidative rearrangement as the terminal step in the anditomin biosynthetic pathway",
            "reactionSMARTS": "[#6@@]123/[#6]/[#6@@]4(-[#6])-[#6]-[#6@@]5\\[#6](=[#8])-[#8]-[#6]\\[#6@@]-1-5-[#6@](\\[#6])(-[#6]-4=[#8])-[#6]-[#6]-2-[#6@]1(/[#6])-[#6](-[#6@@](-[#8]-[#6](=[#8])-[#6]=[#6]-1)(\\[#6])/[#6])-[#6]-[#6]-3>>[#8]=[#6]1-[#6]=[#6]-[#6@]2(-[#6](-[#6]-[#6]-[#6@]34-[#6]-2-[#6@@]2\\[#6](-[#6@]5-3-[#6@@](\\[#6](-[#8]-[#6]\\5)=[#8])-[#6]-[#6@](-[#6]-2=[#8])(-[#6])/[#6]/4)=[#6])-[#6@](/[#6])(\\[#6])-[#8]-1)/[#6]",
            "reactions": [
                {
                    "substrate": "CC1(C)OC(=O)C=C[C@@]2(C)C1CC[C@@]13C[C@@]4(C)C[C@H]5C(=O)OC[C@]51[C@@](C)(CC23)C4=O",
                    "products": [
                        "C=C1[C@@H]2C(=O)[C@]3(C)C[C@@H]4C(=O)OC[C@]14[C@@]1(CCC4C(C)(C)OC(=O)C=C[C@]4(C)C21)C3"
                    ],
                    "isIntermediate": false,
                    "description": "Converts Andilesin C into the final product Anditomin."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1021/ja508127q"
                ]
            }
        }
    ]
}