{
    "accession": "MITE0000359",
    "status": "active",
    "comment": "Uses acetyl-CoA as cofactor.",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-17",
            "contributors": [
                "0000-0002-0771-9611"
            ],
            "reviewers": [
                "0000-0001-6534-6609"
            ],
            "comment": "New entry. Reviewer 1 improved descriptions and added CoA as a cofactor."
        }
    ],
    "enzyme": {
        "name": "AndG",
        "description": "O-acetyltransferase",
        "references": [
            "doi:10.1021/ja508127q"
        ],
        "databaseIds": {
            "uniprot": "A0A097ZPE2",
            "genpept": "BAP81861.1",
            "mibig": "BGC0002612"
        },
        "cofactors": {
            "organic": [
                "Coenzyme A"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Acetylation"
            ],
            "description": "Acetylation of intermediate meroterpenoid in the andilesin biosynthetic pathway.",
            "reactionSMARTS": "[#6@@]123/[#6]/[#6@@]4(-[#6])-[#6@](\\[#8])-[#6@@]5\\[#6](=[#8])-[#8]-[#6]\\[#6@@]-1-5-[#6@](\\[#6])(-[#6]-4=[#8])-[#6]-[#6]-2-[#6@]1(/[#6])-[#6](-[#6@@](-[#8]-[#6](=[#8])-[#6]=[#6]-1)(\\[#6])/[#6])-[#6]-[#6]-3>>[#6@@]123/[#6]/[#6@@]4(-[#6])-[#6@](\\[#8]-[#6](=[#8])-[#6])-[#6@@]5\\[#6](=[#8])-[#8]-[#6]\\[#6@@]-1-5-[#6@](\\[#6])(-[#6]-4=[#8])-[#6]-[#6]-2-[#6@]1(/[#6])-[#6](-[#6@@](-[#8]-[#6](=[#8])-[#6]=[#6]-1)(\\[#6])/[#6])-[#6]-[#6]-3",
            "reactions": [
                {
                    "substrate": "CC1(C)OC(=O)C=C[C@@]2(C)C1CC[C@]13C[C@]4(C)C(=O)[C@](C)(CC21)[C@]31COC(=O)[C@@H]1[C@H]4O",
                    "products": [
                        "CC(=O)O[C@@H]1[C@H]2C(=O)OC[C@@]23[C@]24CCC5C(C)(C)OC(=O)C=C[C@]5(C)C2C[C@@]3(C)C(=O)[C@@]1(C)C4"
                    ],
                    "isIntermediate": true,
                    "description": "Acetylates andilesin A at the C-6' hydroxyl group position, leading to an instable intermediate that eventually results in andilesin B."
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "In vitro assay"
                ],
                "references": [
                    "doi:10.1021/ja508127q"
                ]
            }
        }
    ]
}