{
    "accession": "MITE0000347",
    "status": "active",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-03",
            "contributors": [
                "0009-0003-1714-1678"
            ],
            "reviewers": [
                "0000-0002-7493-459X"
            ],
            "comment": "New entry. Reviewer 1 corrected intermediate product to false and edited description."
        }
    ],
    "enzyme": {
        "name": "PhzF",
        "description": "Isomerase",
        "references": [
            "doi:10.1038/s41598-017-06278-w"
        ],
        "databaseIds": {
            "uniprot": "Q51792",
            "genpept": "AAC18905.1"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Other"
            ],
            "description": "PhzF catalyzes the isomerization of (2\u2009S,3\u2009S)-2,3-dihydro-3-hydroxy anthranilic acid (DHHA) to (1\u2009R,6\u2009S)-6-amino-5-oxo-2-cyclohexene-1-carboxylic acid (AOCHC)",
            "reactionSMARTS": "[#6:1]1-[#6:5]=[#6:6]-[#6@:4](/[#8:10])-[#6@@:2](\\[#7:11])-[#6:3]=1-[#6:7](=[#8:9])-[#8:8]>>[#6:1]1=[#6:5]-[#6:6]-[#6&H0:4](=[#8:10])-[#6@@:2](\\[#7:11])-[#6@@:3]-1/[#6:7](=[#8:9])-[#8:8]",
            "reactions": [
                {
                    "substrate": "N[C@H]1C(C(=O)O)=CC=C[C@@H]1O",
                    "products": [
                        "N[C@@H]1C(=O)CC=C[C@H]1C(=O)O"
                    ],
                    "isIntermediate": false,
                    "description": "PhzF catalyzes the isomerization of (2\u2009S,3\u2009S)-2,3-dihydro-3-hydroxy anthranilic acid (DHHA) to (1\u2009R,6\u2009S)-6-amino-5-oxo-2-cyclohexene-1-carboxylic acid (AOCHC)"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Inference from genomic data and chemical structure",
                    "In vitro assay",
                    "Site-directed mutagenesis"
                ],
                "references": [
                    "doi:10.1038/s41598-017-06278-w"
                ]
            },
            "databaseIds": {
                "rhea": "28182",
                "ec": "5.3.3.17"
            }
        }
    ]
}