{
    "accession": "MITE0000346",
    "status": "active",
    "comment": "Rieske nonheme iron-dependent oxygenase",
    "changelog": [
        {
            "version": "1",
            "date": "2026-05-03",
            "contributors": [
                "0000-0001-9487-1525"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "MarG",
        "description": "Rieske nonheme iron-dependent oxygenase",
        "references": [
            "doi:10.1021/jo5023553",
            "doi:10.1021/ja411544w"
        ],
        "databaseIds": {
            "uniprot": "A0A067YNS9",
            "genpept": "AHF22859.1",
            "mibig": "BGC0000091"
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Cyclization",
                "Heterocyclization",
                "Oxidation"
            ],
            "description": "Cyclization of 23-hydroxyundecylprodiginine to form the macrocycle and spiroaminal moieties of premarineosin A",
            "reactionSMARTS": "[#6:1]-[#6:2](-[#6:4]-[#6:5]-[#6:6]-[#6:7]-[#6:8]-[#6:9]-[#6:10]-[#6:11]-[#6:12]-[#6:13]1:[#7:30]:[#6:16](-[#6:17]=[#6:18]2-[#7:29]=[#6:23](-[#6:24]3:[#7:28]:[#6:27]:[#6:26]:[#6:25]:3)-[#6:22]=[#6:19]-2-[#8:20]-[#6:21]):[#6:15]:[#6:14]:1)-[#8:3]>>[#6:1]/[#6@:2]1-[#8:3]-[#6:18]2(-[#7:29]=[#6:23](-[#6:24]3-[#7:28]-[#6:27]=[#6:26]-[#6:25]=3)-[#6:22]=[#6:19]-2-[#8:20]-[#6:21])-[#6@:17]2/[#6:16]3=[#6:15]-[#6:14]=[#6:13](-[#6:12]-[#6:11]-[#6:10]-[#6:9]-[#6:8]-[#6:7]-[#6:6]\\[#6@@:5]-2-[#6:4]-1)-[#7:30]-3",
            "reactions": [
                {
                    "substrate": "COC1=CC(c2ccc[nH]2)=N/C1=C/c1ccc(CCCCCCCCCC(C)O)[nH]1",
                    "products": [
                        "COC1=CC(c2ccc[nH]2)=NC12O[C@H](C)C[C@H]1CCCCCCCc3ccc([nH]3)[C@@H]12"
                    ],
                    "isIntermediate": true,
                    "description": "Cyclization of 23-hydroxyundecylprodiginine to form the macrocycle and spiroaminal moieties of premarineosin A"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression",
                    "Knock-out studies"
                ],
                "references": [
                    "doi:10.1021/jo5023553",
                    "doi:10.1021/ja411544w"
                ]
            }
        }
    ]
}