{
    "accession": "MITE0000344",
    "status": "active",
    "comment": "Multinuclear nonheme iron-dependent oxidase",
    "changelog": [
        {
            "version": "1",
            "date": "2026-04-20",
            "contributors": [
                "0000-0003-2163-5526"
            ],
            "reviewers": [
                "0000-0002-9747-3423"
            ],
            "comment": "New entry."
        }
    ],
    "enzyme": {
        "name": "HvfB",
        "description": "A multinuclear nonheme iron-dependent oxidase",
        "references": [
            "doi:10.1073/pnas.2408092121"
        ],
        "databaseIds": {
            "uniprot": "Q4QL35",
            "genpept": "AAX88262.1"
        },
        "cofactors": {
            "inorganic": [
                "Fe"
            ]
        }
    },
    "reactions": [
        {
            "tailoring": [
                "Heterocyclization",
                "Oxidation"
            ],
            "description": "Heterocyclization and Oxidation of Cys",
            "reactionSMARTS": "[#7]-[#6]-[#6]-[#6]-[#6]-[#6](-[#6](-[#7]-[#6](-[#6](-[#7]-[#6]-[#6](-[#8])=[#8])=[#8])-[#6]-[#16])=[#8])-[#7]>>[#7]-[#6]-[#6]-[#6]-[#6]-[#6](-[#6]1-[#8]-[#6](=[#8])-[#6](=[#6](-[#7]-[#6]-[#6](-[#8])=[#8])-[#16])-[#7]=1)-[#7]",
            "reactions": [
                {
                    "substrate": "NCCCCC(N)C(=O)NC(CS)C(=O)NCC(=O)O",
                    "products": [
                        "NCCCCC(N)C1=NC(=C(S)NCC(=O)O)C(=O)O1"
                    ],
                    "isIntermediate": true,
                    "description": "Lys-Cys-Gly to oxazolone and thioamide pair"
                }
            ],
            "evidence": {
                "evidenceCode": [
                    "Heterologous expression"
                ],
                "references": [
                    "doi:10.1073/pnas.2408092121"
                ]
            }
        }
    ]
}